Method for enriching and purifying fructooligosaccharide in witloof

A technology of fructooligosaccharides and chicory, which is applied in oligosaccharides, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low extraction purity and low extraction rate, and achieve high extraction purity, large adsorption capacity, and convenient extraction quick effect

Active Publication Date: 2014-03-19
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying fructooligosaccharides in chicory using macroporous adsorption resins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of method utilizing macroporous adsorption resin to enrich and purify fructooligosaccharides in chicory, its steps are as follows:

[0025] (1) After crushing the dried chicory, pass it through a 60-mesh sieve, soak in purified water with 8 times the volume of chicory powder at 50°C for 1 hour;

[0026] (2) Suction-filtering the chicory extract treated in step (1) to obtain the primary fructo-oligosaccharide extract;

[0027] (3) The obtained primary extract is washed with high-concentration ethanol, adjusted to its pH value of 2.0, left standing, suction filtered, and washed with water to neutrality to obtain a crude fructooligosaccharide extract;

[0028] (4) Adsorb the gained fructooligosaccharide crude extract on a macroporous adsorption resin chromatography column, and the macroporous resin used is the NKA-II type resin produced by Tianjin Nankai University;

[0029] (5) Carry out elution in macroporous adsorption resin layer column with the p...

Embodiment example 2

[0032] Implementation case 2: A method for enriching and purifying fructooligosaccharides in chicory using macroporous adsorption resin, the steps are as follows:

[0033] (1) Pass through a 30-mesh sieve after crushing the dried chicory, and soak in purified water with 9 times the volume of chicory powder for 1 hour;

[0034] (2) Suction-filtering the chicory extract treated in step (1) to obtain the primary fructo-oligosaccharide extract;

[0035] (3) The obtained primary extract is washed with high-concentration ethanol, adjusted to pH 2.2, allowed to stand, filtered with suction, and washed with water until neutral to obtain a crude fructooligosaccharide extract;

[0036] (4) the gained fructooligosaccharide crude extract is adsorbed with a macroporous adsorption resin, and the macroporous resin used is the D4006 type resin produced by Tianjin Nankai University;

[0037] (5) Elute the macroporous adsorption resin with 9 times the volume of purified water, track and detect...

Embodiment example 3

[0040] Implementation case 3: A method for enriching and purifying fructooligosaccharides in chicory using a macroporous adsorption resin, the steps are as follows:

[0041] (1) Pass through an 80-mesh sieve after the dried chicory is crushed, and soak in purified water with 10 times the volume of chicory powder for 1.5 hours;

[0042] (2) Suction-filtering the chicory extract treated in step (1) to obtain the primary fructo-oligosaccharide extract;

[0043] (3) The obtained primary extract is washed with high-concentration ethanol, adjusted to pH 2.5, allowed to stand, filtered with suction, and washed with water until neutral to obtain a crude fructooligosaccharide extract;

[0044] (4) the gained fructooligosaccharide crude extract is adsorbed on a macroporous adsorption resin chromatography column, and the macroporous resin used is the NKA-9 type resin produced by Tianjin Nankai University;

[0045] (5) Elute the macroporous adsorbent resin with 10 times the volume of pur...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry and relates to a method for enriching and purifying fructooligosaccharide in witloof by using a macroporous absorption resin. The method has the characteristics of high absorption selectivity on the fructooligosaccharide which is separated and purified by a macroporous absorption technology, high absorption speed and analysis speed, large absorption capacity, convenience and quickness of extraction, abundant sources in raw materials, low production cost, obvious separation effect and high extraction purity. By the method, a fructooligosaccharide semifinished product with content of over 35 percent and a fructooligosaccharide finished product with content of over 95 percent can be obtained, and the defects of relatively low conventional extraction rate and low extraction purity are overcome. The macroporous absorption resin with stable physical and chemical properties, large surface area, high exchange speed, high mechanical strength, high contamination resistance and high thermal stability is adopted; the fructooligosaccharide can be selectively absorbed from the solution through physical absorption; and the absorption speed and the analysis speed are high and the absorption capacity is large.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry and relates to a method for purifying fructooligosaccharides, in particular to a method for enriching and purifying fructooligosaccharides in chicory by using a macroporous adsorption resin. Background technique: [0002] Chicory: A perennial herb of Compositae, with fleshy, short and thick roots. Stem erect, ribbed, hollow, much branched. The leaves are alternate, long oblanceolate, corolla ligule-shaped, and the flower color is blue. The plant is resistant to cold and drought, and likes to be born in sunny fields, hillsides and other places. It is distributed both at home and abroad. Chicory is a dual-purpose plant for medicine and food. Chicory leaves can be used to prepare lettuce. It has been introduced and cultivated in Sichuan (Chengdu) and Guangdong in my country. Its root contains inulin and aromatic substances, which can be used to extract substitute coffee and promote the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C07H1/08
Inventor 姚德坤姚德利闫峰黄运生刘德文姚航郑君
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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