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Nucleic acid quantitative detection kit for hepatitis C virus (HCV)

A hepatitis C virus and detection kit technology, applied in the field of medical detection, can solve the problems of inability to detect non-HCV pathogens, poor specificity of hepatitis C detection kits, etc., to monitor the existence of false negatives, improve detection sensitivity, Good specificity

Active Publication Date: 2013-01-16
SANSURE BIOTECH INC
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0011] The purpose of the present invention provides a quantitative detection kit for hepatitis C virus nucleic acid, which can detect all known 6 genotypes of HCV, but cannot detect non-HCV pathogens, so as to solve the poor specificity of the hepatitis C detection kit in the prior art technical issues

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  • Nucleic acid quantitative detection kit for hepatitis C virus (HCV)
  • Nucleic acid quantitative detection kit for hepatitis C virus (HCV)
  • Nucleic acid quantitative detection kit for hepatitis C virus (HCV)

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Embodiment Construction

[0038] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the claimed invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0039] In a specific embodiment of the present invention, a HCV nucleic acid quantitative detection kit is provided, comprising the following components:

[0040] (1) The probe HCV-P that is used for target polynucleotide detection is derived from the most conserved 5'UTR region of hepatitis C virus and has the sequence of SEQ No.1 (synthesized by Invitrogen):

[0041] SEQ No.1: 5'-ATTTGGGCGTGCCCCCGC-3'

[0042] Preferably, the carboxyl end of SEQ No. 1 is labeled with FAM, and the hydroxyl end is modified with a BHQ1 quenching group. In other embodiments of the present invention, fluorescein labels such as 6-FAM, TET, JOE, and HEX can also...

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Abstract

The invention discloses a nucleic acid quantitative detection kit for a hepatitis C virus (HCV). The kit comprises a probe, an upstream primer, a downstream primer, a PCR (Polymerase Chain Reaction) buffer solution and DNA (Deoxyribonucleic Acid) polymerase, wherein the probe is used for detecting target polynucleotide; the upstream primer and the downstream primer are used for amplifying the target polynucleotide; and the probe for detecting the target polynucleotide has a sequence shown in SEQ No.1. Because the probe for detecting the target polynucleotide has the sequence shown in SEQ No.1, the kit has high specificity. Due to the application of the kit, the concentration of HCV-RNA (Hepatitis C Virus-Ribonucleic Acid) in unknown samples such as serum, blood plasma or milky juice and the like can be measured quickly and accurately, and six genotypes of all HCVs can be detected.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a hepatitis C virus nucleic acid quantitative detection kit. Background technique [0002] Hepatitis C is distributed worldwide, and the population is generally susceptible. The infection rate of hepatitis C virus (Hepatitis C Virus, HCV) is 0.1%-10%, with an average of 3%. About 170-200 million people in the world are infected with HCV. The HCV infection rate of the general population in my country is 3%, about 38 million infected persons. 70-80% of HCV-infected patients develop chronic hepatitis C, and the manifestations of chronic hepatitis C also vary in severity. About 20-30% can gradually develop into liver cirrhosis. Among patients with liver cirrhosis, about 1%-4% develop into liver cirrhosis every year. develop liver cancer. [0003] Anti-HCV enzyme-linked immunosorbent reagents are currently the main means of diagnosing hepatitis C. They are generally used in the ins...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 戴立忠
Owner SANSURE BIOTECH INC
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