Method and device for constructing human auricular cartilage in vitro

Abstract

The invention relates to a method and device for constructing the human auricular cartilage in vitro. At present, the human auricular cartilage is statically cultured in vitro by adopting manual plasticity, only an auricular cartilage model of small scale is constructed and the normal human auricular cartilage can not be constructed. The device comprises an auricular cartilage bioreactor, a control unit and a measurement unit, wherein the human auricular cartilage bioreactor comprises a cam rotating mechanism, a movable guide mechanism, a displacement regulation mechanism, an auricular cartilage culture mechanism and a positioning linking mechanism; the control unit comprises a touch screen, a programmable controller, a motor and a speed regulator; and the measurement unit comprises a pressure sensor, a flow sensor, a data acquisition card and measurement software. The invention realizes fully-automatic dynamic culture of the human auricular cartilage by adopting a pressure stress and perfusion way; and the human auricular cartilage bioreactor can be massively cultured and constructed in a manner of combining one device into multiple heads, and previous manual operation and static culture modes are completely changed.

Description

technical field [0001] The patent of the invention belongs to the field of biomedical engineering, and relates to a method and device for dynamically culturing human auricles in vitro, in particular to a method and device for compounding seed cells with biodegradable materials and perfusing them in dynamic environments of different frequencies and compressive stresses. A culture device for human auricle with cell viability, good human auricle morphology and certain mechanical strength. It is suitable for constructing human auricular cartilage with good shape in vitro in the field of biomedical engineering. Background technique [0002] When the tissue engineering method is used to combine cells and biodegradable materials to construct tissue-engineered human auricle cartilage, the shape of the human auricle constructed in vitro is far from that of a normal person, and the internal tissue of the human auricle cartilage is easy to form a hollow, etc. Compared with normal huma...

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Problems solved by technology

[0002] When the tissue engineering method is used to combine cells and biodegradable materials to construct tissue-engineered human auricle cartilage, the shape of the human auricle constructed in vitro is far from that of a normal person, and the internal tissue of the human auricle cartilage is easy to form a hollow, etc. Defects, the mechanical properties are far from normal human auricular cartilage, and cannot meet the requirements of clinical transplantation

The current method of cultivating the human auricle is manual plasticity and static culture in vitro, which can only construct a small-scale human auricle model, and cannot realize the normal construction of normal human auricle cartilage

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