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Method for detecting mannitol content of cordyceps hawkesii

A detection method and mannitol technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of poor specificity, prone to errors, large errors, etc., and achieve the effect of good reproducibility and improved quality

Active Publication Date: 2013-05-08
山西省药品检验所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For the determination of mannitol, the commonly used methods include volumetric method and colorimetric method, but the disadvantage of the volumetric method is that it is limited to the determination of pure products. If it is a preparation or a collection of multiple compounds, the reducing substances in the components can also be detected by iodine. The result is that the measured content is higher than the actual value; and the disadvantage of the colorimetric method is that it has poor specificity and is prone to errors; since the content of mannitol in Cordyceps sinensis varies from place to place, the TLC scanning method is used To measure, the error is large
[0008] Because the content of mannitol in Cordyceps sinensis is different from place to place, for the unique Cordyceps sinensis in Shanxi, the above several methods cannot efficiently and accurately measure the content of mannitol. In order to solve this problem, the present invention uses gas phase Chromatographic Determination of Mannitol in Shanxi's Unique Cordyceps Cordyceps

Method used

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  • Method for detecting mannitol content of cordyceps hawkesii

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The detection method of mannitol content in Cordyceps sinensis comprises the following steps:

[0031] (1) Preparation of reference substance solution: Accurately weigh 30 mg of mannitol reference substance dried at 100°C to constant weight in a 10ml volumetric flask, add 5ml of pyridine-acetic anhydride with a volume ratio of 1:1, and place in a water bath at 100°C React for 40 minutes, let it cool down, and dilute to the mark with pyridine, shake well to get the reference substance solution;

[0032] (2) Preparation of the test solution: Grind and sieve the Cordyceps sinensis, accurately weigh 1g, add absolute ethanol to a Soxhlet extractor and extract for 5.5 hours until it is nearly colorless, and the extract is recovered to dryness, and the volume ratio of the residue is 40ml of 1:1 pyridine-acetic anhydride was acetylated in a water bath at 100°C for 40 minutes, let it cool, then transferred to a 50ml volumetric flask, dilute to the mark with pyridine, and shake w...

Embodiment 2

[0035] The detection method of mannitol content in Cordyceps sinensis comprises the following steps:

[0036] (1) Preparation of reference substance solution: Accurately weigh 30 mg of mannitol reference substance dried at 100°C to constant weight in a 10ml volumetric flask, add 5ml of pyridine-acetic anhydride with a volume ratio of 1:1, and place in a water bath at 100°C React for 40 minutes, let it cool down, and dilute to the mark with pyridine, shake well to get the reference substance solution;

[0037] (2) Preparation of the test solution: Grind and sieve the Cordyceps sinensis, accurately weigh 1 g, add absolute ethanol to a Soxhlet extractor and extract for 6.5 hours until it is nearly colorless, and the extract is recovered to dryness, and the volume ratio of the residue is 40ml of 1:1 pyridine-acetic anhydride was acetylated in a water bath at 100°C for 40 minutes, let it cool, then transferred to a 50ml volumetric flask, dilute to the mark with pyridine, and shake ...

Embodiment 3

[0040] The detection method of mannitol content in Cordyceps sinensis comprises the following steps:

[0041] (1) Preparation of reference substance solution: Accurately weigh 30 mg of mannitol reference substance dried at 100°C to constant weight in a 10ml volumetric flask, add 5ml of pyridine-acetic anhydride with a volume ratio of 1:1, and place in a water bath at 100°C React for 40 minutes, let it cool down, and dilute to the mark with pyridine, shake well to get the reference substance solution;

[0042] (2) Preparation of the test solution: Grind and sieve the Cordyceps sinensis, accurately weigh 1g, add absolute ethanol to a Soxhlet extractor and extract for 6 hours until it is nearly colorless, and the extract is recovered to dryness, and the ratio of residue to volume is 40ml of 1:1 pyridine-acetic anhydride was acetylated in a water bath at 100°C for 40 minutes, let it cool, then transferred to a 50ml volumetric flask, dilute to the mark with pyridine, and shake well...

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Abstract

The invention discloses a method for detecting mannitol content of cordyceps hawkesii, and belongs to the technical field of Chinese medicinal component content measurement. The mannitol content of the cordyceps hawkesii is measured by using gas chromatography, and the efficient and accurate measurement of the mannitol content of the special cordyceps hawkesii in Shanxi is realized. The method comprises the following steps of: preparing reference solution; preparing sample solution; and performing measurement by adopting a gas chromatograph, wherein the chromatography conditions comprise that: the initial temperature of column temperature is 220 DEG C and kept for 1 minute, the temperature is raised to between 220 and 230 DEG C at the rate of 10 DEG C per minute, the raised temperature iskept for 26 minutes, the temperature of a vaporizing chamber and the temperature a hydrogen flame ionization detector are 300 DEG C, the flow rate of H2 is 34 to 40ml / min, the flow rate of air is 350to 400ml / min, the flow rate of N2 is 30 to 35 / min, and the split ratio is 50:1. The method is simple, convenient, quick and accurate, has good repeatability, can measure the mannitol content of the special cordyceps hawkesii in Shanxi more scientifically and accurately, and effectively improves the quality of a product.

Description

technical field [0001] The invention belongs to the technical field of content determination of traditional Chinese medicine components, and in particular relates to a detection method for the content of mannitol in Cordyceps sinensis. Background technique [0002] The modernization of Chinese medicine standards is an important part of the modernization of Chinese medicine, and the modernization of Chinese medicinal material standards is the basis for the modernization of Chinese medicine standards. Selecting the iconic chemical components in the main taste of Chinese patent medicines as index components and measuring their content is the main method for chemically determining the content of Chinese patent medicines; the traditional Chinese medicines recorded in the invention and general drug standards (especially "Chinese Pharmacopoeia") The content determination method of the index components contained in the material is consistent with the content determination method of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/68
Inventor 樊开珍施怀生郭景文梁宏朴晋华李琛连云岚张俊凤
Owner 山西省药品检验所
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