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Application of gambogic acid in preparing inflammation inhibitor

A technology of inflammation inhibitor, gambogic acid, applied in the field of gambogic acid, can solve the problems of unreported anti-inflammatory physiological activity and so on

Inactive Publication Date: 2011-09-28
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Studies have shown that it has a certain curative effect in clinical treatment of tumors, but it has not been reported that it has anti-inflammatory physiological activity

Method used

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  • Application of gambogic acid in preparing inflammation inhibitor
  • Application of gambogic acid in preparing inflammation inhibitor
  • Application of gambogic acid in preparing inflammation inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 gambogic acid cytotoxicity test

[0020] The cytotoxicity of gambogic acid was detected by MTT method, and the specific steps were as follows:

[0021] RAW264.7 cells (1×10 6 cells / well) were inserted into a 96-well culture plate and cultured overnight, adding bacterial lipopolysaccharide (lipopolysaccharide, LPS) (100ng / ml) and different concentrations of gambogic acid (10, 100, 500, 800nmol / L, using DMSO as a blank Control) act together for 24h or different concentrations of gambogic acid (10, 100, 500, 800nmol / L) act alone for 24h, then add MTT to the final concentration of 0.5mg / ml and act for 3h, add the dissolving solution (10% SDS, 0.01mol / L HCL) measure OD after overnight 590 .

[0022] OD 590 Can reflect the growth of cells, so by comparing OD 590 Value to judge whether gambogic acid has cytotoxicity.

[0023] The results of the MTT assay showed that (see figure 1 and 2 ): Gambogic acid has basically no cytotoxicity to RAW264.7 cells under ...

Embodiment 2

[0024] Example 2 Application of Gambogic Acid in Inhibiting RAW264.7 Cell Inflammation Response Induced by Bacterial Lipopolysaccharide

[0025] By detecting the changes in the concentration of pro-inflammatory factors in cells, it can be determined whether gambogic acid can inhibit the inflammatory response of RAW264.7 cells. The specific test method is as follows:

[0026] 1) Set the gambogic acid concentration gradient. RAW264.7 cells (4×10 5 cells / mL) into a 24-well culture plate, after 24 hours, add LPS to a final concentration of 100ng / ml to stimulate for 6 hours, and at the same time add different concentrations of gambogic acid (10, 100, 300, 800nmol / L, use DMSO as a blank control) , to observe the effect of gambogic acid on the expression of inflammatory factors before LPS stimulation. Through ELISA detection kit instructions (purchased from eBioscience Company, Mouse TNF-α (Cat#: 88-7324-88, Lot#: E09483-1334) and Mouse IL-6 (Cat#: 88-7064-88, Lot# : E09362-1631)...

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Abstract

The invention relates to an application of gambogic acid in preparing an inflammation inhibitor, in particular to an application of the gambogic acid in preparing medicaments for treating inflammatory factor mediated inflammatory diseases such as endotoxin shock and the like. The gambogic acid has a chemical formula of C38H44O8 and a molecular weight of 628.75, and can be used for preparing the medicaments for treating the inflammatory factor mediated inflammatory diseases such as endotoxin shock and the like, wherein the inflammatory diseases comprise rheumatic arthritis, inflammatory bowel disease, neurodegenerative disease, septic shock and the like. Approved by pharmacological experiments, the gambogic acid can effectively inhibit inflammatory factors such as tumor necrosis factor (TNFalpha), interleukin 6 (IL-6) and nitric oxide (NO), and can be used as an inflammation inhibitor for preparing the medicaments for treating the inflammatory factor mediated inflammatory diseases such as rheumatic arthritis, inflammatory bowel disease, neurodegenerative disease, septic shock and the like.

Description

technical field [0001] The present invention relates to a gambogic acid (Gambogic acid, abbreviated as GBA), in particular to the application of gambogic acid in the preparation of inflammation inhibitors. Background technique [0002] Inflammatory response is one of the important components of the body's immune system, which is precisely regulated by the body through various channels. However, when the inflammatory response is uncontrollable, it will lead to various diseases in the body, such as rheumatoid arthritis (rtheumatoid arthritis), inflammatory bowel disease (Inflammatory bowel disease, IBD), neurodegenerative disease (neurodegenerative disorder) and septic shock (septic shock syndrome) and so on, long-term inflammatory response stimulation can also induce body canceration, which seriously threaten people's physical and mental health (1, Monaco, et al., Curr Drug Targets Inflamm Allergy, 2004, 3, 35-42 ; 2, Schwartsburd et al., Cancer Metastasis Rev, 2003, 22, 95-...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P25/28A61P29/00A61P19/02A61P1/00
Inventor 张连茹耿晶易玉婷郑忠辉宋思扬黄耀坚
Owner XIAMEN UNIV
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