Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dual fluorescence quantification RT-PCR detection kit and application thereof

A technology of RT-PCR and detection kits, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of wasting clinical specimens and reagents, time-consuming and laborious, and save clinical specimens and Effects of Reagents, Improving Influenza Testing, Increased Biosecurity Risk

Active Publication Date: 2013-03-06
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the increasing importance of human seasonal influenza, avian influenza and H1N1 influenza in public health, it is time-consuming, laborious and wasteful of clinical specimens and reagents to detect multiple influenza viruses at the same time during influenza surveillance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dual fluorescence quantification RT-PCR detection kit and application thereof
  • Dual fluorescence quantification RT-PCR detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Materials and methods

[0042] Virus strains and clinical specimens:

[0043] Influenza A H1N1, SWH1N1, seasonal H1N1, and seasonal H3N1 virus strains were isolated from Zhejiang Provincial Center for Disease Control and Prevention. The clinical samples were obtained from the throat swabs of suspected patients with the recent outbreak of H1N1 virus in Zhejiang Province. The samples were collected and transported to the laboratory with ice.

[0044] 1.2 Primers and probes

[0045] A(H1N1), seasonal H1N1, porcine H1N1 influenza virus strains from all over the world were downloaded from the NCBI gene bank in the United States. Homology comparisons were carried out, and influenza A H1N1-specific primers and Taqman probes were designed in the HA gene region of all influenza virus genomes. The sequences are as follows:

[0046] Upstream primer alpha-infA-FP: 5’- GACCRATCYTGTCACCTCTGAC-3’

[0047] Downstream primer alpha infA-RP: 5'-AGGGCATTYTGGACAAAKCGTCTA-3'

[0048]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a double fluorescent quantitative RT-PCR detection kit, comprising deoxynucleoside triphosphate mixture, MgCl2, RNase inhibitor, Moloney murine leukemia virus reverse transcriptase, DNA polymerase, influenza virus standard , Reference substance. The present invention provides a rapid, specific, accurate and sensitive genetic diagnosis method and diagnostic kit for molecular biology of multiplex fluorescent quantitative PCR based on the sequence analysis of the currently popular influenza A (H1N1) virus. It can detect and distinguish influenza A virus or influenza B virus at the same time for 2 hours, perfecting influenza detection.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a double fluorescence quantitative RT-PCR detection kit for influenza A virus and influenza B virus nucleic acid and an application thereof. Background technique [0002] Since March 18, 2009, there has been an outbreak of human infection with H1N1 influenza virus in Mexico. Since the first death case of human infection with influenza A H1N1 virus appeared in Mexico on April 13, 2009, as of May 5, 21 countries around the world have confirmed the existence of influenza A (H1N1) influenza cases through laboratory testing. The number of cases rose to 1,124, including 26 deaths. In addition to Mexico and the United States, many countries have reported confirmed cases of influenza A (H1N1). To this end, the World Health Organization (WHO) raised the global influenza pandemic warning level to level 4, and further raised it to level 5 on April 29, which means that in one of the WHO'...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 张严峻
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com