HCM (Hypertrophic Cardiomyopathy) genotyping method and kit
A technology of hypertrophic cardiomyopathy and genotyping method, which is applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as unfavorable factors in treatment, and achieve the effect of preventing spread
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Embodiment 1
[0043] A hypertrophic cardiomyopathy gene PCR detection kit provided by the present invention includes primer base sequences for PCR amplification and sequencing of gene exons of MYH7, MYBPC3 and TNNT2, and also includes PCR amplification reaction solution: 1 μL of 10× PCR Buffer, 1μL of 2μM primers, 1μL of 2mM dNTP mix, 1μL of 15mM MgCl 2 , 1U Taq enzyme, 1μL 20ng / μL DNA template, add ddH 2 O to make up the volume to 10 μL.
[0044] The PCR primer sequences of the exons of the MYH7 gene are shown in Table 1, and the reference genome sequence is the sequence with the accession number NM_000364.1 in the GenBank database (the reference sequence of this patent has been corrected).
[0045] The PCR primer sequences of the exons of the MYBPC3 gene are shown in Table 2, and the reference genome sequence is the sequence whose accession number is U91629.1 in the GenBank database.
[0046] The PCR primer sequences of the exons of the TNNT2 gene are shown in Table 3, and the reference g...
Embodiment 2
[0056] 1. PCR-sequencing method to detect mutations in the exons of the MYH7 gene
[0057] The subjects of the study were 80 patients with hypertrophic cardiomyopathy who were clearly diagnosed in the hospital, and they had no blood relationship through family investigation. Among them, there were 40 probands in families with hypertrophic cardiomyopathy, 285 related family members, and 40 sporadic cases. 80 normal study controls were healthy people matched in sex and age.
[0058] Take 3 mL of EDTA anticoagulated venous blood from the patient, and divide it into 1.5 mL tubes, 300 μL per tube. Add 500 μL red blood cell lysate (10mM PH=7.5 Tris-Cl, 0.32M sucrose, 1% Triton-X-100, 5mM MgCl) to each tube 2 ), centrifuge at 9000rpm for 30s after mixing, and discard the supernatant. Repeat the above steps 2-3 times until the supernatant becomes clear. Add 300 μL leukocyte lysate (10 mM pH=8.0 Tris-Cl, 400 mM NaCl, 2 mM pH8.0 Na 2 EDTA), and 40 μL of 10% SDS, shaken for 30 s, su...
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