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Differential expression map model of long-chain non-coding RNA in uremia and its construction method

A long-chain non-coding, ribonucleic acid technology, applied in the field of differential expression model of uremic ribonucleic acid, can solve problems such as lack of research results and technical information

Active Publication Date: 2011-12-21
中国人民解放军联勤保障部队第九二四医院
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Problems solved by technology

[0005] However, there are no specific research results and technical information on how to combine lncRNA methods for comprehensive analysis of uremia

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  • Differential expression map model of long-chain non-coding RNA in uremia and its construction method
  • Differential expression map model of long-chain non-coding RNA in uremia and its construction method
  • Differential expression map model of long-chain non-coding RNA in uremia and its construction method

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Embodiment Construction

[0022] The specific implementation manners of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] The present invention uses lncRNA chip technology to detect the differences in lncRNA expression profiles in peripheral blood mononuclear cells of healthy people and patients with uremia, screens out differentially expressed lncRNA, uses real-time quantitative PCR technology to further verify the chip results, and obtains peripheral blood mononuclear cells of uremia. The differential expression map model of long-chain non-coding RNA in cells assists in the study of uremia. like figure 1 As shown, one embodiment of the present invention is a method for constructing a pattern model of differential expression of long-chain non-coding ribonucleic acid in peripheral blood mononuclear cells of uremia, which includes the following steps:

[0024] A1. Obtain the isolated test peripheral blood samples of the uremia patient group ...

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Abstract

The invention relates to a construction method of a uremia peripheral blood mononuclear cell long-chain non-coding ribonucleic acid difference expression spectrum model, which comprises the following steps of: A1, obtaining the in vitro test peripheral blood samples of the uremia patient group and the normal control group and respectively obtaining gene chip data for each test peripheral blood sample; and A2, uploading the gene chip data of each test peripheral blood sample to gene chip data analysis software, setting the normalized standard and data filtering ratio times, obtaining the difference expression gene and obtaining the uremia peripheral blood mononuclear cell long-chain non-coding ribonucleic acid difference expression spectrum model. Through the scheme, the information as themiddle results is provided for the study on the uremia, and in addition, a novel idea is provided for further disclosing the pathological mechanism of the uremia. In addition, the invention also relates to the uremia peripheral blood mononuclear cell long-chain non-coding ribonucleic acid difference expression spectrum model.

Description

【Technical field】 [0001] The invention relates to a differential expression model of ribonucleic acid in uremia, in particular to a differential expression map model of long-chain non-coding ribonucleic acid in peripheral blood mononuclear cells of uremia and a construction method thereof. 【Background technique】 [0002] Uremia refers to a series of symptoms of self-intoxication in the body when acute or chronic renal insufficiency develops to a severe stage. Uremia is generally related to genetic and environmental factors, but these factors cannot fully elucidate the mechanism of uremia. Inflammatory cytokines induced by acute kidney injury may contribute to the development of kidney disease. Some studies have found that iron, ferritin, C-reactive protein (CRP), tumor necrosis factor (INF), interleukin 10 (IL-10) and interleukin 6 (IL-6), etc. are related to uremia. In the absence of renal parenchymal damage, potential cytokine mRNAs affecting uremia were searched. Curre...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/68
Inventor 眭维国戴勇刘娟
Owner 中国人民解放军联勤保障部队第九二四医院
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