Methanol flowing feeding on-line disinfection method and automatic control system

Abstract

The invention discloses a methanol flowing feeding on-line disinfection method and an automatic control system, which solve the problems of potential safety hazard existence, bacterium contamination, difficult automatic control and the like. Through the arrangement of relevant equipment and steps of residual air pipeline methanol cleaning, steam pipeline disinfection, methanol slag removal, sterilization, automatic flowing feeding and the like, the treatment process is distinct, the pertinence is high, integral structural components for realizing the flow process are simple, various problems caused by automatic methanol flowing feeding in pichia pastoris fermentation are effectively solved, and a high use value is realized.

Description

Technical field

[0001] The invention relates to the field of fermentation, more specifically to the fed-batch process of methanol in the fermentation process. Background technique

[0002] The Pichia pastoris expression system can use the strong alcohol oxidase 1 (AOX1) promoter to achieve high-efficiency expression of foreign genes under the induction of methanol. Compared with natural strains and E. coli expression systems, it has a high expression level , good stability, low culture cost, easy separation of products, high product safety and many other fermentation advantages, using Pichia pastoris to express foreign proteins and produce a variety of biological products has great commercial value, solving the problem of Pichia pastoris fermentation The problems in the process and equipment are of great significance.

[0003] Pichia pastoris methanol-induced fermentation generally uses a two-step process. The engineered bacteria are first amplified in the medium with glyc...

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