Methanol flowing feeding on-line disinfection method and automatic control system

A disinfection method and methanol technology, applied in the field of fermentation, can solve problems such as unseen facilities and solutions, and achieve the effect of high use value, strong pertinence and clear processing flow

Inactive Publication Date: 2013-01-16
淮北市三和诺生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] At present, due to comprehensive factors such as potential safety hazards, bacterial contamination, and difficulty in automatic control in the process of feeding methanol in China, the industrialization of Pichia pastoris fermentation has been greatly restricted, and there is no domestic solution to properly solve the above problems. Facilities and Solutions

Method used

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  • Methanol flowing feeding on-line disinfection method and automatic control system

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Embodiment Construction

[0027] As shown in the drawings, the present invention includes several control valves, a methanol tank 1, a water storage tank 2, a methanol pre-filter 3, a methanol sterilization filter 4, a peristaltic pump 5, a fermenter 6, a peristaltic pump control component 7, etc. Control valve F1, control valve F2, methanol pre-filter 3, methanol sterilization 4, peristaltic pump 5, control valve F4 are sequentially connected between methanol tank 1 and fermenter 6; the control valve F1 is also passed through the control valve F9 is connected to the water storage tank 2, a branch is set between the methanol pre-filter 3 and the methanol sterilization filter 4 to connect the control valve F3, the control valve F4 is also connected to the control valve F5, and passes through the control valve F6 The control valve F7 and the control valve F8 are connected, and the peristaltic pump 5 is connected with the automatic control part 7 .

[0028] The control components include transmitters, PLC...

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Abstract

The invention discloses a methanol flowing feeding on-line disinfection method and an automatic control system, which solve the problems of potential safety hazard existence, bacterium contamination, difficult automatic control and the like. Through the arrangement of relevant equipment and steps of residual air pipeline methanol cleaning, steam pipeline disinfection, methanol slag removal, sterilization, automatic flowing feeding and the like, the treatment process is distinct, the pertinence is high, integral structural components for realizing the flow process are simple, various problems caused by automatic methanol flowing feeding in pichia pastoris fermentation are effectively solved, and a high use value is realized.

Description

Technical field [0001] The invention relates to the field of fermentation, more specifically to the fed-batch process of methanol in the fermentation process. Background technique [0002] The Pichia pastoris expression system can use the strong alcohol oxidase 1 (AOX1) promoter to achieve high-efficiency expression of foreign genes under the induction of methanol. Compared with natural strains and E. coli expression systems, it has a high expression level , good stability, low culture cost, easy separation of products, high product safety and many other fermentation advantages, using Pichia pastoris to express foreign proteins and produce a variety of biological products has great commercial value, solving the problem of Pichia pastoris fermentation The problems in the process and equipment are of great significance. [0003] Pichia pastoris methanol-induced fermentation generally uses a two-step process. The engineered bacteria are first amplified in the medium with glyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/12C12M1/04C12M1/36
CPCC12M41/00C12M37/02
Inventor 吴友标
Owner 淮北市三和诺生物工程有限责任公司
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