Method for distinguishing back muscles from muscles of three parts of male cyprinus carpio

A muscle, back technology, used in biological testing, material inspection products, etc.

Inactive Publication Date: 2012-04-04
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
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Problems solved by technology

However, studies that can solve this problem with protein detection methods have not been reported.

Method used

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with embodiment.

[0020] Materials: GAPDH protein antibody, mouse secondary antibody, and rabbit secondary antibody were purchased from Jill Biochemical (Shanghai) Co., Ltd.

[0021] 1. The five-tailed male carp was taken from the Yixing Breeding Base of Wuxi Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences. The back muscles, abdominal muscles and tail muscle tissues were taken, and they were quickly frozen in liquid nitrogen and stored in a -80°C refrigerator for easy generalization. RNA extraction. The extraction of total RNA was carried out according to the operating instructions of RNAiso Plus, and dissolved in DEPC water after the extraction was completed. The extraction of RNA was detected by agarose gel electrophoresis, the concentration of RNA was detected by spectrophotometry, and then the RNA was reverse-transcribed into cDNA with a reverse transcription kit for...

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Abstract

The invention relates to a method for distinguishing back muscles from muscles of three parts of a male cyprinus carpio. The method comprises the following steps of: first obtaining a high-quality cyprinus carpio bone morphogenetic protein (BMP) 4 antibody; then successfully extracting proteins of the back muscles, belly muscles and tail muscles of the male cyprinus carpio; and finally detecting BMP4 proteins in muscle tissues of a target cyprinus carpio by using the detected cyprinus carpio BMP4 antibody, taking glyceraldehydes-3-phosphate dehydrogenase (GAPDH) as a control of target proteins, determining that the muscles belong to the back muscles of the male cyprinus carpio if the proteins of 55KD can be detected, and determining that the muscles belong to the muscles of the other parts of the male cyprinus carpio if the proteins of the 55KD cannot be detected. The method has the advantages that: the back muscles can be distinguished from the belly muscles and tail muscles of the male cyprinus carpio; a breakthrough can be provided for researches on pectoral muscle distinguishing and the other muscles; and simultaneously, a way and a method for the breeding of the back muscles of the male cyprinus carpio can be sought according to a molecular marker.

Description

technical field [0001] The invention relates to a method for distinguishing back muscles from three different parts of male carps. Background technique [0002] At present, consumers agree that among the back muscles, abdominal muscles, and tail muscles of carp, the back muscles are fresh and tender, which is favored by consumers. Like this, in the process of carp production and breeding, the selection and formation mechanism of back muscle becomes the primary problem that must be solved. Therefore, how to distinguish the dorsal muscles of male carp at the molecular level is particularly important. [0003] Molecular markers for identifying back muscles can be developed from the back muscles, abdominal muscles, and tail muscles of male carps, which can provide a breakthrough for research on the difference between pectoral muscles and other muscles. paths and methods. However, studies that can solve this problem with protein detection methods have not been reported. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 苏胜彦董在杰张成锋袁新华徐跑
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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