TRAP (Telomeric Repeal Amplification Protocol)-SCAR (Sequence Characterized Amplified Region) 424 marker for identifying E genome of agropyron elongatum and application of marker

Abstract

The invention discloses a TRAP (Telomeric Repeal Amplification Protocol)-SCAR (Sequence Characterized Amplified Region) 424 marker for identifying E genome of agropyron elongatum and application of the marker. The nucleotide sequence of the marker is shown by SEQ.ID.NO.3 or SEQ.ID.NO.6. A specific TRAP segment is obtained by using a TRAP-SCAR method through screening and is converted into a stable SCAR marker; and the amplification of the stable SCAR marker is detected. According to the marker for the E genome of the agropyron elongatum which is provided by the invention, through the detection of the SCAR marker, favorable foundation for identifying E chromatin or genome of the agropyron elongatum under the genetic background of wheat is laid; and meanwhile, a very convenient path for molecular marker assistant breeding is provided.

Description

Technical field [0001] The present invention belongs to the technical field of identification of E. longiflorum chromosomes of group E, and relates to a TRAP-SCAR for identifying the E group chromosomes of Elytrigia longiflora 424 Marking and its application. Background technique [0002] Wheat is one of the most important food crops in the world. It is the second largest crop in China after rice, and wheat production has a very important strategic significance for the development of the national economy. Due to the simplification of wheat cultivars, its genetic basis is becoming increasingly narrow and the source of resistance is scarce, which greatly limits the further improvement of its yield and quality. There are a large number of genetic variations in the relative species of wheat, which are beneficial gene resources that can be used for wheat improvement. The introduction of these beneficial genes into wheat has always been an enduring research topic for geneticists and b...

AI Technical Summary

Problems solved by technology

[0004] Hybridization and introgression are an effective way to introduce the excellent traits and genes of exogenous chromosomes into wheat. The detection of chromatin or chromosomes has become a crucial issue. Although valuable genes have been mapped on the chromosomes of E. elongatum through agronomically important characteristics and biochemical analysis, the detection methods of these experiments are not stable, and Not suitable for testing important agronomic traits

Some genetic methods, such as chromosome banding and in situ hybridization, have been used to detect exogenous genetic material in the genetic background of wheat, but these techniques are time-consuming, laborious, and have high technical requirements. These experimental methods still have certain limitations for large-scale rapid screening of offspring.

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