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Method for electric measurement of peroxide using CNT sensor

A peroxide, sensor technology, applied in nanotechnology for sensing, material analysis by electromagnetic means, instruments, etc., can solve the problems of complicated operation and spectroscopic measurement device, etc.

Inactive Publication Date: 2012-05-23
HOKKAIDO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the thiobarbituric acid method requires complicated operations and spectroscopic measurement devices

Method used

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  • Method for electric measurement of peroxide using CNT sensor
  • Method for electric measurement of peroxide using CNT sensor
  • Method for electric measurement of peroxide using CNT sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0079] 1. Fabrication of CNT sensors

[0080] (1) Preparation of substrate

[0081] The region other than the electrode formation predetermined part of the glass substrate (20 mm x 20 mm) was covered with a resist film by the photolithography method. Titanium and gold films are formed by vapor deposition at the predetermined positions for electrode formation to form electrodes with a two-layer structure. After that, the resist film is removed.

[0082]On the other hand, 1 mL of 30% hydrogen peroxide aqueous solution was added in small portions to 3 mL of sulfuric acid to prepare a sulfuric acid / hydrogen peroxide mixed liquid. The glass substrate on which the electrodes were formed was put into a 20 mL beaker, and the sulfuric acid / hydrogen peroxide mixed solution was dropped while cooling the entire beaker. Incubate for 10 minutes at room temperature with gentle agitation. After 10 minutes, the glass substrate was washed with deionized water, followed by washing with pure ...

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PUM

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Abstract

Disclosed is a method for measuring the concentration of a peroxide using a CNT sensor. The CNT sensor comprises a working electrode that is arranged on an insulating substrate, a monolayered carbon nano-tube that is contacted with the working electrode, a counter electrode, and a reference electrode. A sample is provided on the monolayered carbon nano-tube, and a potential difference is made between the working electrode and the counter electrode. In this manner, the concentration of the peroxide in the sample can be measured. The measurement method can be applied to clinical tests or the like.

Description

technical field [0001] The present invention relates to a method for the electrical determination of peroxides using a CNT sensor. Background technique [0002] Biochemical examination using hydrogen peroxide-peroxidase is one of the basic methods of clinical examination. For example, in the examination of blood sugar, neutral fat, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, fatty acid, uric acid (causative substance of gout), creatinine (indicator of kidney function), etc., In the first step, which is different for each measurement object, hydrogen peroxide is generated by a reaction using an enzyme specific to the measurement object, and in the common second process, hydrogen peroxide is generated by using a peroxidase-based reaction Hydrogen peroxide generated in the first step was measured spectroscopically. In addition, in immunoassays, hydrogen peroxide-peroxidases are also used as one of the indicators for evaluating the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/30
CPCB82Y15/00G01N27/3278
Inventor 千叶仁志武田晴治石井睦中村基训武笠幸一惠淑萍黑泽隆夫
Owner HOKKAIDO UNIVERSITY