Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof

A technology of flower jujube honey and licorice polysaccharide is applied in the field of traditional Chinese medicine formula granule health food, which can solve the problems such as the blank in the research and development field of licorice polysaccharide.

Active Publication Date: 2012-06-27
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems in the prior art, the present invention provides a licorice polysaccharide, honeysuckle and jujube honey granul

Method used

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  • Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof
  • Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof
  • Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0020] Preparation of licorice polysaccharide

[0021] (1) Obtaining of licorice aseptic seedlings: select licorice seeds full of particles and dark green, stir and sterilize the seeds on a magnetic stirrer with 10% sodium hypochlorite, the rotating speed is 300r / min, and the temperature is 25°C. Sterilization time was 30 minutes, MS culture contained 3% sucrose, pH 5.9 before sterilization, inoculated in a 50ml Erlenmeyer flask containing MS solid medium, placed in a light incubator at 25°C, and cultivated for 30 days to obtain licorice sterilized seedlings.

[0022] (2) Acquisition of licorice callus: the licorice callus was induced from the hypocotyls of the aseptic seedlings of licorice, and after several times of selection and subculture, the growth state was stable. The subculture medium of callus was MS medium, supplemented with 2,4-D (1 mg·L -1 ), NAA (1mg·L -1 ), 6-BA (0.2mg·L -1 ), 3% sucrose, pH 5.9 before sterilization, subculture cycle 28 days.

[0023] (3) Ob...

Embodiment 1

[0026] Extraction of licorice polysaccharide: take 500g licorice cell powder, add 20 times the weight of distilled water, let it stand for 12 hours, extract in a water bath at 100°C for 1 hour, repeat the extraction 3 times, combine the filtrates, concentrate to a certain volume, add 4 times the volume of absolute ethanol, Stand overnight at 4°C, remove the supernatant, remove protein by sevage method, and dry in vacuum to obtain licorice polysaccharide powder.

[0027] Preparation of honeysuckle extract: extract honeysuckle with water 3 times, add 10 times the weight of the medicinal material each time, extract for 1 hour each time, extract at 80°C, concentrate and dry the extract to form extract.

[0028] Preparation of jujube extract: Extract the jujube powder 3 times with water, the amount of each extraction is 15 times that of the raw medicinal material, the extraction time is 1.5 hours each time, the extraction temperature is 95°C, the extract is concentrated and dried to...

Embodiment 2

[0031] Extraction of licorice polysaccharide: Take 500g licorice cell powder, add 15 times the weight of distilled water, let it stand for 8 hours, extract in a water bath at 100°C for 2 hours, repeat the extraction twice, combine the filtrates, concentrate to a certain volume, add 4 times the volume of absolute ethanol, Stand overnight at 4°C, remove the supernatant, remove protein by sevage method, and dry in vacuum to obtain licorice polysaccharide powder.

[0032] Preparation of honeysuckle extract: extract honeysuckle with water once, adding water 15 times the weight of the medicinal material each time, extraction time is 3 hours each time, extraction temperature is 90°C, and the extract is concentrated and dried to form an extract.

[0033] Preparation of jujube extract: Extract the jujube powder with water twice, adding 10 times the amount of water each time as the raw medicinal material, extraction time is 2.5 hours each time, extraction temperature is 80°C, and the ext...

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Abstract

The invention relates to glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof. The glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules are mainly made of, by weight, 8%-12% of glycyrrhiza polysaccharide, 2%-6% of honeysuckle extract, 2%-6% of Chinese date extract, 1%-3% of honey, and 73%-87% of accessories. The health granules mainly made of the glycyrrhiza polysaccharide with the honeysuckle, the Chinese date, the honey and the like bridge the gap of the glycyrrhiza polysaccharide in terms of health product development, and have the effects of immunity improvement and fatigue resistance. The method extracting glycyrrhizae suspension cells to obtain the glycyrrhiza polysaccharide is insusceptible to seasons and regions and is convenient and easy in operation.

Description

technical field [0001] The invention relates to a health food and a preparation method thereof, in particular to a Chinese medicine formula granule health food. Background technique [0002] As a traditional Chinese medicine, licorice has been known as "ten directions and nine herbs" since ancient times. The main active ingredients of licorice are glycyrrhizic acid, licorice brass and licorice polysaccharide. Functional research mainly focuses on anti-tumor, anti-virus, regulation of immune activity and scavenging free radicals, and there is little development in the application of licorice polysaccharide. As a new way to produce licorice polysaccharides, licorice suspension cells have more potential for development. The content of licorice polysaccharides produced by it is higher than that of wild licorice, and it has obvious anti-oxidation, anti-virus, anti-tumor, and immunomodulatory activities; Lonicera japonica Thunb. Dried buds or with first blooms of Lonicera japonic...

Claims

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Application Information

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IPC IPC(8): A23L1/08A23L2/39A23L21/25
Inventor 高文远郭松波满淑丽王仁芳刘辉王娟
Owner TIANJIN UNIV
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