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Transformed cryX gene and application thereof

A gene and expression vector technology, applied in the new insecticidal gene cryX and its application fields, can solve the problems of increasing pest resistance and singleness, etc.

Active Publication Date: 2013-06-19
北京中植科创生物技术有限公司
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AI Technical Summary

Problems solved by technology

[0006] Since the current commercialized transgenic insect-resistant crops have relatively single types of insect-resistant genes, such large-scale promotion of planting has the risk of reducing pest refuges and increasing pest resistance.

Method used

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  • Transformed cryX gene and application thereof
  • Transformed cryX gene and application thereof
  • Transformed cryX gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] Determination of insecticidal activity of strain T03B001 (preservation number: BGSC No.4 AP1, the public can obtain from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences)

[0037] The strain was inoculated on LB solid medium and cultivated for 72 hours until the spores and crystals were released. Wash the spores and crystals with sterilized water, suspend them, and dilute to a concentration of 100 million spores per milliliter for the determination of insecticidal activity. The insecticidal activity against Lepidoptera pests was determined with diamondback moth, corn borer and cotton bollworm. The results showed that the spore crystal mixture with this concentration had high activity against diamondback moth, corn borer and cotton bollworm. The mortality rate of the three tested insects was 100%.

[0038] Design primers to amplify the full-length cryX gene

[0039] The T03B001 genome was sequenced using the Solexa method.

[0040] Gene scr...

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PUM

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Abstract

The invention relates to a transformed cryX gene and application of the transformed cryX gene, which belongs to the field of biotechnology. The transformed cryX gene has a sequence shown as SEQ ID NO3 and is applied to transform microorganisms or plants so that the microorganisms or plants express toxicity to related pests and overcomes and postpones the generation of drug resistance of pests on engineering bacteria. The experiment shows that the maximum content of the expressed CryX protein accounts for 0.21% of total soluble proteins.

Description

[0001] This application is a divisional application with the patent application number "201010196386X" and the invention title "insecticidal cryX gene with high toxicity to lepidopteran pests and its application". technical field [0002] The invention relates to the field of biotechnology, in particular to a novel insecticidal gene cryX with high toxicity of bacillus thuringiensis to lepidopteran pests and its application. Background technique [0003] Insect pests are one of the important reasons for crop yield reduction, and reducing the loss of insect pests is an important way to increase the yield of food and feed crops. According to statistics, the total output of food and forage crops in the world is lost to 14% every year due to pests, and the economic losses directly caused by agricultural production are as high as hundreds of billions of dollars. In my country, the annual loss caused by insect pests is 10% reduction in rice production, 20% reduction in wheat produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/32C12N15/63C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 耿丽丽束长龙刘东明张杰宋福平黄大昉
Owner 北京中植科创生物技术有限公司
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