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Method for preparing phosphatidylserine-enriched krill oil

A technology rich in phosphatidylserine and shrimp oil, applied in the direction of fat oil/fat production, fat production, etc., to achieve the effect of simple operation and simple process

Inactive Publication Date: 2012-07-11
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports of extracting phosphatidylserine from Antarctic krill.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] a. Thaw frozen Antarctic krill, then add Antarctic krill to the ethanol solution according to the ratio of 500g Antarctic krill and 750mL ethanol solution with a weight concentration of 90%, extract 4 times under stirring, each time for 6h, and combine the extraction liquid and filtered to obtain the filtrate;

[0014] b. Concentrate the filtrate of step a to 11% of the volume of the combined filtrate at 60°C and -0.09MPa to obtain a concentrated extract;

[0015] c. Add the same volume of n-hexane to the concentrated extract in step b, mix well, let it stand still, immediately separate the four layers with clear boundaries, and then take the second layer of liquid from top to bottom;

[0016] d. Evaporate the liquid taken out in step c to remove n-hexane at 55° C. and a pressure of -0.07 MPa to obtain light yellow shrimp oil. The shrimp oil contains 8% phospholipids, of which PS accounts for 41% of the phospholipids.

Embodiment 2

[0018] a. Thaw frozen Antarctic krill, then add Antarctic krill to the ethanol solution according to the ratio of 500g Antarctic krill and 1250mL ethanol solution with a weight concentration of 100%, extract twice under stirring, each time for 8h, and combine the extraction liquid and filtered to obtain the filtrate;

[0019] b. Concentrate the filtrate of step a to 12% of the volume of the combined filtrate at 65°C and -0.07MPa to obtain a concentrated extract;

[0020] c. Add the same volume of n-hexane to the concentrated extract in step b, mix well, let it stand still, immediately separate the four layers with clear boundaries, and then take the second layer of liquid from top to bottom;

[0021] d. Evaporate the liquid taken out in step c to remove n-hexane at 50° C. and a pressure of -0.09 MPa to obtain light yellow shrimp oil. The shrimp oil contains 10% of phospholipids, and PS accounts for 40% of the phospholipids.

Embodiment 3

[0023] a. Thaw frozen Antarctic krill, then add Antarctic krill to the ethanol solution according to the ratio of 500g Antarctic krill and 1000mL ethanol solution with a weight concentration of 95%, extract 3 times under stirring, 7h each time, and combine the extraction liquid and filtered to obtain the filtrate;

[0024] b. Concentrate the filtrate of step a to 11.5% of the volume of the combined filtrate at 63°C and -0.08MPa to obtain a concentrated extract;

[0025] c. Add the same volume of n-hexane to the concentrated extract in step b, mix well, let it stand still, immediately separate the four layers with clear boundaries, and then take the second layer of liquid from top to bottom;

[0026] d. Evaporate the liquid taken out in step c to remove n-hexane at 52° C. and a pressure of -0.08 MPa to obtain light yellow shrimp oil. The shrimp oil contains 9% phospholipids, of which PS accounts for 40.05% of the phospholipids.

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PUM

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Abstract

The invention provides a method for preparing phosphatidylserine-enriched krill oil. The method comprises the following steps of: a) unfreezing frozen Neptune krill, adding each 1kg of Neptune krill into 1.5 to 2.5L of 90 to 100 weight percent ethanol solution, extracting for 2 to 4 times with stirring, extracting for 6 to 8 hours each time, mixing extracting solutions, and filtering to obtain filtrate; b) concentrating the filtrate obtained in the step a) at the temperature of between 60 to 65DEG C and the pressure of between -0.07 and -0.09MPa until the filtrate is 11 to 12 volume percent of the mixed filtrate, thus obtaining a concentrated extracting solution; c) adding n-hexane in the same volume as the concentrated extracting solution into the concentrated extracting solution obtained in the step b), uniformly mixing, standing for demixing, and taking the second layer of liquid from top to bottom; and d) evaporating the liquid taken in the step c) at the temperature of between 50and 55DEG C and the pressure of between -0.07 and -0.09MPa to remove the n-hexane to obtain the product.

Description

technical field [0001] The invention relates to the technical fields of medicine, chemicals, food and health food, and further relates to a method for preparing shrimp oil rich in phosphatidylserine from Antarctic krill. Background technique [0002] Antarctic krill live in the Antarctic freshwaters of the Antarctic Ocean and live in clusters. There are about 500 million tons, and now the Antarctic krill fishery in the world is about 100,000 tons per year. It is a rich overseas resource that my country will exploit in recent years. Frozen Antarctic krill generally contains about 80% water. The phospholipid content in Antarctic krill oil is relatively high. The main components of phospholipids are phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI). , phosphatidic acid (PA) and phosphatidylserine (PS). According to data records, the content of phosphatidylserine (PS) in Antarctic krill phospholipids is very low, accounting for about 2% of ...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/10
Inventor 刘代成
Owner SHANDONG NORMAL UNIV
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