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Application of mTOR (mammalian Target of Rapamycin) serving as target to screening of medicament for treating nicotine addiction

An addictive drug, nicotine technology, applied in the field of medicine to achieve the effect of preventing relapse

Inactive Publication Date: 2012-10-03
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it plays a key role in nicotine addiction and whether it can be used as a target for smoking cessation drugs has not been reported

Method used

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  • Application of mTOR (mammalian Target of Rapamycin) serving as target to screening of medicament for treating nicotine addiction
  • Application of mTOR (mammalian Target of Rapamycin) serving as target to screening of medicament for treating nicotine addiction
  • Application of mTOR (mammalian Target of Rapamycin) serving as target to screening of medicament for treating nicotine addiction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Effects of a single administration of nicotine on the p70s6k phosphorylation activity of the nucleus accumbens shell and basolateral amygdala (BLA) of the mesolimbic dopamine system in rats

[0020] p70s6k is a downstream activation protein of mTOR, which controls protein synthesis, and its activation state can reflect the activity of mTOR.

[0021] Materials and Methods

[0022] Drugs and reagents Nicotine: sigma-N0267; DMSO and other reagents are commercially available analytical reagents.

[0023] Animal SPF grade male SD rats, weighing 220-250g. Provided by the Animal Experiment Center of Wuhan University, the animal certificate number is NO.00014833, and the production license number is SCXK (E) 2003-2004. Rat feed was purchased from the Experimental Animal Center of Wuhan University.

[0024] experimental method

[0025] Animal grouping and treatment: Rats were randomly divided into normal saline and nicotine administration groups. All administrati...

Embodiment 2

[0032] Example 2: Effects of Nicotine Receptor Antagonists on Changes in the Phosphorylation Activity of the Nucleus Accumbens Shell and BLA of the Mesolimbic Dopamine System in Rats

[0033] In this example, Mecamylamine was selected as the antagonist of the anti-nicotine effect, and whether Mecamylamine could weaken the activation of mTOR by nicotine on the nucleus accumbens shell.

[0034] Materials and Methods

[0035] Drugs and reagents Mecamylamine: sigma-M9020; DMSO and other reagents are commercially available analytical reagents.

[0036] Animal SPF grade male SD rats, weighing 220-250g. Provided by the Animal Experiment Center of Wuhan University, the animal certificate number is NO.00014833, and the production license number is SCXK (E) 2003-2004. Rat feed was purchased from the Experimental Animal Center of Wuhan University.

[0037] experimental method

[0038] Animal grouping and treatment: the rats were randomly divided into 4 groups, namely the normal salin...

Embodiment 3

[0045] Example 3: The effect of localized injection of mTOR inhibitor rapamycin on the formation of nicotine-conditioned place preference in nucleus accumbens shell

[0046] In this example, the rat conditioned place preference formation model is selected as an animal model for detecting nicotine addiction and Rapamycin is used as a preparation for inhibiting the activation effect of nicotine on mTOR, to explore whether Rapamycin can inhibit the mTOR of nicotine on the nucleus accumbens shell Activates and inhibits the formation of a conditioned place preference (i.e., the formation of an addiction).

[0047] Materials and Methods

[0048] Drugs and reagents Mecamylamine: sigma-M9020; DMSO and other reagents are commercially available analytical reagents.

[0049] Animal SPF grade male SD rats, weighing 220-250g. Provided by the Animal Experiment Center of Wuhan University, the animal certificate number is NO.00014833, and the production license number is SCXK (E) 2003-2004....

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PUM

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Abstract

The invention discloses an application of mTOR (mammalian Target of Rapamycin) serving as a target to screening of a medicament for treating nicotine addiction. The mTOR is taken as a target for preparing a medicament for treating nicotine substance addiction. An effective medicament acting target is provided for the treatment of symptoms such as nicotine substance addiction and excitability, and can be used for radically treating nicotine substance addiction.

Description

technical field [0001] The invention relates to the application of mTOR as a target in screening and treating nicotine addiction drugs, and belongs to the technical field of medicine. Background technique [0002] Smoking is harmful to health and has become a public health issue of concern to the whole society. Smoking can cause diseases such as cancer, cardiovascular and cerebrovascular diseases, respiratory tract and digestive tract diseases. The number of deaths due to smoking in the world reaches more than 5 million every year, and tobacco-related diseases account for the Cause of death comes first. According to the "2007 China Smoking Control Report" reported by the Ministry of Health: China is the world's largest tobacco producer and consumer, with tobacco production accounting for one-third of the world's total. By the end of 2006, there were 350 million smokers in my country. Accounting for one-third of the total number of smokers in the world, there are 540 million ...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 李艳琴彭淑娴温泉林觉郑春明
Owner WUHAN UNIV
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