Kit for evaluating chemotherapy effect of lung adenocarcinoma

A kit, a technology for lung adenocarcinoma, which is applied in the biological field to achieve the effect of simple, easy and reliable expression level

Inactive Publication Date: 2014-07-02
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In patients with lung cancer, the response rate to a single chemotherapy or antineoplastic drug treatment is generally lower than that of other diseases
In addition, the effective concentration of chemotherapy drugs is often close to or even coincides with the toxic concentration

Method used

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  • Kit for evaluating chemotherapy effect of lung adenocarcinoma

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Experimental program
Comparison scheme
Effect test

Embodiment

[0018] A kit for evaluating the effect of lung adenocarcinoma chemotherapy, consisting of cytokines and their enzyme-labeled antibodies, carbonate buffer solution with a pH value of 9.6, phosphate buffer solution with a pH value of 7.4, serum protein diluent, stop solution, tetramethylbenzidine substrate solution, normal human serum and positive control serum;

[0019] Wherein, the cytokines include chemokine ligand 21, chemokine ligand 28, eotaxin-3, hemofiltration CC chemokine-1, leukemia inhibitory factor, macrophage 10 kinds of cytokines including macrophage inflammatory protein-3a, neutrophil chemoattractant protein-2, human recombinant macrophage inflammatory protein-4, and stromal cell-derived factor-1α.

[0020] The carbonate buffer solution with a pH value of 9.6 was obtained by adding 1.59 g of sodium carbonate and 2.93 g of sodium bicarbonate to 1 L of distilled water. The phosphate buffer solution with a pH value of 7.4 is that 0.2 gram of potassium dihydrogen pho...

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Abstract

The invention discloses a kit for evaluating the chemotherapy effect of lung adenocarcinoma. The kit is characterized in that the kit comprises cell factors and enzyme-labeled antibody thereof, carbonate buffer solution with the pH value of 9.6, phosphate buffer solution with the pH value of 7.4, serum protein dilute solution, stop solution, tetramethyl benzidine substrate solution, normal human serum and positive control group serum, wherein the cell factors comprises 10 sorts of cell factors including chemotactic factors, inflammation relevant factors, immunological cell factors and the like. The kit can assist on evaluating the chemotherapy effect of lung adenocarcinoma.

Description

technical field [0001] The invention relates to a kit for evaluating the chemotherapy effect and function of lung adenocarcinoma, which belongs to the field of biotechnology. Background technique [0002] Lung cancer is a common cancer worldwide and one of the leading causes of death in cancer patients. The morbidity and mortality of lung cancer rank first among all malignant tumors in my country. According to the statistics of The International Agency for Research on Cancer (IARC) of the World Health Organization, the incidence of lung cancer in my country in 2008 was 35 per 100,000 people, and the agency estimated that by 2025, there will be 1 million new lung cancer cases in my country every year. example. According to the 2010 Health Statistical Yearbook of the Ministry of Health, as of 2009, the national lung cancer mortality rate was 30.83 / 100,000 people, which was higher than the second highest mortality rate of liver cancer by more than 4 / 100,000 people. Lung cance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
Inventor 白春学王向东杨达伟
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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