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Reagent kit for diagnosing chronic diastolic heart failure

A heart failure and kit technology, applied in the biological field, can solve problems such as limitation, influence and change of cardiac filling mode, transient changes, etc., and achieve the effect of simple, easy and reliable expression level

Inactive Publication Date: 2010-06-02
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

However, there are many objective factors that lead to the obvious limitations of these diagnostic methods, such as non-specific or transient changes in cardiac load status, age changes, changes in heart rate, or the presence of mitral regurgitation, etc., will affect the filling pattern of the heart. influence and change
Current studies have found that serum levels of brain natriuretic peptide (BNP) are helpful to the diagnosis of diastolic heart failure to a certain extent, but this diagnostic method is also limited because BNP levels are affected by other factors such as age

Method used

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  • Reagent kit for diagnosing chronic diastolic heart failure
  • Reagent kit for diagnosing chronic diastolic heart failure

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Embodiment

[0020] A kit for diagnosing chronic diastolic heart failure, consisting of cytokines and their enzyme-labeled antibodies, pH9.6 carbonate buffer, pH7.4 phosphate buffer, serum protein diluent, stop solution, tetramethyl Base benzidine substrate solution, normal human serum and positive control serum.

[0021] The cytokines are 17 kinds of cytokines including growth factors, inflammation-related factors, neuron trophic factors, and blood coagulation factors.

[0022] The pH9.6 carbonate buffer is obtained by adding 1.59 g of sodium carbonate and 2.93 g of sodium bicarbonate to 1 L of distilled water.

[0023] The PH7.4 phosphate buffer solution is 0.2 grams of potassium dihydrogen phosphate, 2.9 grams of disodium hydrogen phosphate dodecahydrate, 8.0 grams of sodium chloride, 0.2 grams of potassium chloride, and 0.5ml of Tween-20 added to 1L of distilled water And get.

[0024] The serum protein diluent is obtained by adding 0.1 gram of bovine serum, goat serum or rabbit seru...

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Abstract

The invention provides a reagent kit with the function of forecasting chronic diastolic heart failure, which is characterized by comprising cell factors and an enzyme labelled antibody thereof, a carbonate buffer solution with pH being 9.6, a phosphate buffer solution with pH being 7.4, a serum protein diluent, a stop solution, a tetramethyl benzidine substrate solution, a normal human serum and a positive control serum, wherein the cell factors are 17 cell factors which comprise a growth factor, a phlogosis correlation factor, a neuron trophic factor, a coagulation factor and various other cell factors. The invention can assist the diagnosis of chronic diastolic heart failure.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit with the function of predicting chronic diastolic heart failure. Background technique [0002] Heart failure is a complex clinical syndrome caused by structural or functional abnormalities of the heart that impair ventricular filling and ejection ability, and is the terminal stage in the development of various clinical cardiovascular diseases. Heart failure has received increasing attention because of its high morbidity and mortality. In the United States, the overall incidence rate is about 1.5% to 2.0%, and among people aged ≥65, it is as high as 6% to 10%. According to a random survey in my country, the incidence rate of adult heart failure is 0.9% (0.7% for men and 1.0% for women), and the 5-year survival rate of heart failure patients is only 35%. In addition, the medical expenses of heart failure are increasing year by year. According to relevant statistics, the hospitali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53G01N21/31
Inventor 姜红葛均波王向东张磊刘明
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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