Reagent kit for diagnosing non-small cell lung cancer

A technology of non-small cell lung cancer and a kit, applied in the biological field, can solve the problems of reducing the mortality rate of lung cancer patients, the effect of the mortality rate of lung cancer patients is not significant, and achieve the effect of simple, feasible and reliable expression level

Inactive Publication Date: 2015-05-20
ZHONGSHAN HOSPITAL FUDAN UNIV
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AI Technical Summary

Problems solved by technology

Although imaging and cytology screening are used for early detection of lung cancer, although they have certain sensitivity, they have little effect on reducing the mortality rate of lung cancer patients; and the development of specific cytokine markers as a supplement to imaging technology may reduce the incidence of lung cancer patients. fatality rate

Method used

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  • Reagent kit for diagnosing non-small cell lung cancer
  • Reagent kit for diagnosing non-small cell lung cancer

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Experimental program
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Embodiment

[0018] A test kit for diagnosing non-small cell lung cancer, characterized in that it consists of cytokines and their enzyme-labeled antibodies, carbonate buffer solution with a pH value of 9.6, phosphate buffer solution with a pH value of 7.4, serum protein dilution solution, stop solution, tetramethylbenzidine substrate solution, normal human serum and positive control serum;

[0019] Wherein, the cytokines include chemokine ligand 21, tyrosine protein kinase 7, β-cell factor, chemokine ligand 28, skin T cell capture chemokine, granulocyte chemoattractant protein 2, human Long-regulated oncogene, hemofiltration CC chemokine 1, hemofiltration CC chemokine-2, interleukin-9, interleukin-18-binding protein a, leukemia inhibitory factor, can compete with glycoprotein D for herpesvirus entry into T TNF-like inducible protein at cellular site, monocyte chemoattractant-3, monocyte chemoattractant-4, macrophage-derived cytokine, macrophage-stimulating protein alpha, neutrophil chemoa...

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Abstract

The invention discloses a reagent kit for diagnosing a non-small cell lung cancer. The reagent kit for diagnosing the non-sugar cell lung cancer is characterized by comprising cytokines and an enzyme-labeled antibody thereof, a carbonate buffer with the pH value of 9.6, a phosphate buffer with the pH value of 7.4, a serum protein diluent, a stopping solution, a tetramethylbenzidine substrate solution, a normal human serum and a positive control serum, wherein the cytokine comprises 23 cytokines including chemokine, a inflammation-related factors, immunocytokine and the like. The reagent kit for diagnosing the non-small cell lung cancer can assist the diagnosis of the non-small cell lung cancer.

Description

technical field [0001] The invention relates to a kit for diagnosing the function of non-small cell lung cancer, which belongs to the field of biotechnology. Background technique [0002] Lung cancer is a common cancer worldwide and one of the leading causes of death in cancer patients. The morbidity and mortality of lung cancer rank first among all malignant tumors in my country. According to the statistics of The International Agency for Research on Cancer (IARC) of the World Health Organization, the incidence of lung cancer in my country in 2008 was 35 per 100,000 people, and the agency estimated that by 2025, there will be 1 million new lung cancer cases in my country every year. example. According to the 2010 Health Statistical Yearbook of the Ministry of Health, as of 2009, the national lung cancer mortality rate was 30.83 / 100,000 people, which was higher than the second highest mortality rate of liver cancer by more than 4 / 100,000 people. Lung cancer is generally di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
Inventor 白春学王向东杨达伟
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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