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Chromatography-mass spectrometry analysis of co stable isotope mass number abundance ratio method

A stable isotope and mass number technology, applied in the analysis of materials, measuring devices, material separation, etc., can solve problems such as single, not enough to express the source of carbon monoxide in detail, and achieve the effect of simple method and good application prospects

Active Publication Date: 2015-10-07
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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Problems solved by technology

However, in animals and plants with rich sources of carbon monoxide, it is obvious that a carbon monoxide 13 C / 12 The stable isotope ratio of C is too simple to express the source of carbon monoxide in detail

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  • Chromatography-mass spectrometry analysis of co stable isotope mass number abundance ratio method
  • Chromatography-mass spectrometry analysis of co stable isotope mass number abundance ratio method
  • Chromatography-mass spectrometry analysis of co stable isotope mass number abundance ratio method

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Embodiment Construction

[0024] Chromatography-mass spectrometry analysis CO stable isotope mass number abundance ratio method, comprising the following steps:

[0025] (1) Establish a mathematical model for the stable isotope ratio of carbon monoxide:

[0026] According to the carbon monoxide molecule is composed of five stable isotopes 12 C. 13 C. 16 O. 17 O. 18 O element composition, under different conditions, 6 kinds of carbon and oxygen elements with different mass numbers will be formed, as shown in Table 1:

[0027]

[0028] Accordingly, the mass abundance ratios of six stable isotopes of carbon monoxide can be obtained, namely Table 2:

[0029] .

[0030] (2) Pass the carrier gas with a purity greater than 99.999% through the purifier at an output pressure of 0.20~0.60 MPa, so that the purified carrier gas has a purity greater than 99.9999% or greater than 99.99999%.

[0031] (3) Pass the purified carrier gas into the chromatographic column of the gas chromatograph where the sampl...

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Abstract

The invention relates to a method for analyzing the CO stable isotope mass number abundance ratio through chromatography-mass spectrometry. The method comprises the following steps: (1) building a CO stable isotope ratio mathematical model; (2) filling a carrier gas with the purity of 99.999% into a purifier, and obtaining the purified carrier gas with the purity of over 99.9999% or over 99.99999%; (3) filling the purified carrier gas into a chromatographic column which contains a sample to be measured and is arranged in a gas chromatograph, after the adsorption and desorption, measuring CO in the sample to be measured through a mass detector, and obtaining the mass numbers of 28, 29, 30 and 31 of CO in the gas to be measured are obtained; and (4) putting the mass numbers of 28, 29, 30 and 31 of CO in the gas to be measured, and obtaining 6 CO isotope mass number abundance ratios. The method realizes the purpose of finely expressing the CO source through building the mathematical model, and can be applied to non-traumatic detection of human health, human body medicine and animal and botanical researches.

Description

technical field [0001] The invention relates to the field of carbon monoxide stable isotope research, in particular to a method for analyzing the mass number abundance ratio of CO stable isotope by chromatography-mass spectrometry. Background technique [0002] Carbon and oxygen stable isotopes in carbon monoxide molecules are tracers for the study of ecosystem and atmospheric material circulation and flux. Previously in this field by detecting carbon monoxide 13 C / 12 The stable isotope ratio of C is used to identify the source of carbon monoxide. However, in animals and plants with rich sources of carbon monoxide, it is obvious that a carbon monoxide 13 C / 12 The stable isotope ratio of C is too simple to compare, and it is not enough to express the source of carbon monoxide in detail. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a simple and easy chromatography-mass spectrometry method for analyzing CO...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 潘旭
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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