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Method for quantitatively detecting ATP, ADP, and AMP in tissue

A quantitative detection and organization technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of inaccurate results, cumbersome operation, and long detection time, and achieve the effects of high sensitivity, simple operation, and cheap medicines

Inactive Publication Date: 2013-01-02
谈善军 +1
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AI Technical Summary

Problems solved by technology

At present, the content of ATP, ADP, and AMP in the detection tissue is relatively high, but most methods require a long time for detection, cumbersome operation, and inaccurate results

Method used

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Examples

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Embodiment Construction

[0006] Reagents needed in the present invention are: 5'-adenosine triphosphate disodium salt, 5'-adenosine diphosphate disodium salt, 5' adenosine monophosphate disodium salt (SIGMA company of the United States); Methanol (mobile phase B) is chromatographic Pure; ultrapure water (made in the laboratory, resistivity ≥ 16MΩ.cm); perchloric acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, and potassium hydroxide are all analytically pure.

[0007] Then configure the solutions in order: ①Configuration of the standard solution: use 4°C pre-cooled ultrapure water to configure 5 standard solution with concentration gradients (the concentrations of ATP, ADP, and AMP of the sample to be tested are in the standard solution. concentration range) ②Preparation of phosphate buffer solution (mobile phase A): phosphate buffer solution with a concentration of 100mmol / L (containing 12mmol / L disodium hydrogen phosphate and 88mmol / L sodium dihydrogen phosphate), and 2mol / L potassiu...

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Abstract

The invention relates to a substance detection method, specifically a method for quantitatively detecting ATP, ADP, and AMP in tissue. According to precedence order, the method includes preparing reagent-preparing solution-setting chromatographic condition-making standard curve-preparing solution of sample to be detected-measuring content of specimen. The method is suitable for wide range, applicable for quantitative measurement of ATP, ADP, and AMP in the fields of clinical examination, scientific research, medicolegal expertise, etc.; the method can detect the content of ATP, ADP, and AMP in tissue with high efficiency, has the advantages of high sensitivity, high stability, high repeatability, high precision, and high recovery rate; the method can separate ATP, ADP, and AMP in tissue within 10 minutes, effectively reduce the interference of impurities; the reagent and chemicals used in the detection method are cheap, can be easily prepared, and have low cost, the invention has rapid detection and short time, facilitates to detect in batch, and is simple for operation.

Description

technical field [0001] The invention relates to a substance detection method, especially a method for quantitative detection of tissue ATP, ADP and AMP. Background technique [0002] 5′-Adenosine 5′-triphosphate (ATP) is the direct source of energy in the body and has an important impact on the physiological functions of the human body. The reduction of ATP will lead to insufficient energy supply, etc., which will lead to various physiological dysfunctions and even the occurrence of diseases. In addition, ATP is an important indicator for identifying the time of death in forensic research. Adenosine 5'-diphosphate (ADP) and Adenosine 5'-monophosphate (AMP) are degradation products of ATP, which play an important role in the balance of ATP in the body. In addition, studies have found that increased ADP and AMP may lead to increased fatigue. Therefore, quantitative detection of ATP, ADP, and AMP content in tissues is of great significance for clinical diagnosis and treatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 余震谈善军
Owner 谈善军
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