Quality testing method for fingerprint of herbal medicine musk

A quality detection method and fingerprint technology are applied in the field of fingerprint quality detection of Chinese medicinal material musk, which can solve problems such as the quality decline of musk, reflection of musk quality, scarcity and the like.

Active Publication Date: 2013-02-06
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the continuous scarcity of musk resources and human factors, the quality of musk has declined, and it is difficult to objectively reflect the quality of musk only by using existing national standards.

Method used

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  • Quality testing method for fingerprint of herbal medicine musk
  • Quality testing method for fingerprint of herbal medicine musk
  • Quality testing method for fingerprint of herbal medicine musk

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0039] The selection of experimental example 1 need testing product solution extraction solvent

[0040] Get natural musk (batch number: TR1#), carry out ultrasonic extraction to natural musk TR1# respectively with ether, dehydrated alcohol, adopt HP-1 chromatographic column, analyze on gas chromatograph (Agilent 7890A), use gas chromatography-mass spectrometry A combined instrument (Agilent 7890A / Agilent 5975C) was used to assist in the identification, and the peak numbers and the chromatographic peak areas of the two main components in musk (macrocyclic ketones and steroids) within the peak elution time range were compared. The results show that when absolute ethanol is used as the extraction solvent, the chromatograms have more information in the peak time range of the two types of components; while diethyl ether is used as the extraction solvent, although the amount of information in the peak time range of the macroketones is relatively large. There are many, but the amoun...

experiment example 2

[0041] The selection of experimental example 2 need testing product solution extraction method

[0042] Take about 0.2 g of natural musk (batch number: TR1#) dried under reduced pressure, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 10 ml of absolute ethanol, seal it tightly, weigh it, shake it for 2 minutes, press Extraction in the following four experimental schemes: A. Sonicate in an ice bath for 5 minutes, place it at room temperature, make up the weight with reagents, shake well, and let it stand overnight; B. Sonicate in an ice bath for 15 minutes, place it at room temperature, make up the weight with reagents, and shake well ; C, standing for 2 hours; D, standing for 4 hours. The experimental results show that the two schemes A and B are relatively complete. Since scheme B takes the shortest time and saves time, the scheme B is selected for ultrasonication in an ice bath for 15 minutes, placed at room temperature, supplemented with reagent...

experiment example 3

[0043] Experimental example 3 selection of gas chromatography column

[0044] Using absolute ethanol and diethyl ether as extraction solvents, 10 batches of musk musk were ultrasonically extracted using the extraction scheme B of Experimental Example 2, and sampled for analysis. The following three chromatographic columns were tested: ①HP-1 elastic quartz capillary column 30m×0.25mm×0.25μm, ②DB-1701 elastic quartz capillary column 30m×0.32mm×0.25μm, ③HP-5 elastic quartz capillary column 30m×0.32 mm×0.25μm, analyzed on an Agilent GC-6890N gas chromatograph, and summarized the data using the number of chromatographic peaks as an index. The results are shown in Table 2.

[0045] Table 2 Comparison of the total peak numbers of the domestic musk ether extract and alcohol extract on different chromatographic columns

[0046]

[0047] The results show that, using absolute ethanol as a solvent to ultrasonically extract 10 batches of musk musk, the total number of peaks obtained by...

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Abstract

The invention discloses a quality testing method for fingerprint of herbal medicine musk. The method includes establishing a gas chromatographic fingerprint of the musk by gas chromatography, and analyzing chemical components of different types of musk by gas chromatography and mass spectrometry. A test sample is processed by specific process, chromatographic detection is optimized, and tests show that the method is stable, precise and repeatable. Three established reference musk fingerprints are well specific. Similarity of the three types of musk is researched under integration of non-integration conditions of muscone, and more accurate basis is provided for testing quality of the three different types of musk. In addition, common compounds of the three types of musk are obtained by identification by GC-MS (gas chromatography and mass spectrometry) and can be used as basis for further identification of synthetic musk, domestic musk and natural musk.

Description

technical field [0001] The invention relates to a method for detecting the quality of fingerprints of Chinese medicinal materials, in particular to a method for detecting the quality of fingerprints of musk, a Chinese medicinal material. Background technique [0002] The quality standard of musk is included in the 2010 edition of the Chinese Pharmacopoeia, and the national standard stipulates the inspection of properties, purity, loss on drying, total ash and the content of muscone by gas chromatography. However, due to the continuous scarcity of musk resources and human factors, the quality of musk has declined, and it is difficult to objectively reflect the quality of musk only by using existing national standards. Therefore, it is very necessary to establish a quality detection method that can more comprehensively reflect the volatile components in musk. Contents of the invention [0003] The purpose of the invention is to establish a method for detecting the quality o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 潘杰陈纪鹏于娟夏松
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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