Application of composition of Chinese yarn polysaccharide and Chinese yarn protein
A technology of yam protein and yam polysaccharide, which is applied in the direction of drug combination, peptide/protein composition, medical preparations containing active ingredients, etc.
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Embodiment 1
[0015] a. Use the dried rhizome of yam crude drug as raw material, remove plant impurities, weigh 10 kg and pulverize it, heat it with water twice the weight of yam at a temperature of 50°C, reflux extraction twice for 2 hours, filter, and combine the extracts , concentrate the extract to 50% after filtering;
[0016] b. Add 95% ethanol to the concentrated solution to make the alcohol content of the concentrated solution reach 30%, and remove the precipitate after precipitation occurs;
[0017] c. Continue to add 95% ethanol to the concentrated solution to make the alcohol content reach 90%. After the precipitate appears, let it stand for 3 hours. After the precipitate is fully precipitated, remove the supernatant to collect the precipitate, then dry it and crush it. , the yellow-brown powder yam polysaccharide content of 40-50% and the composition of yam protein content of 30-40% can be obtained.
Embodiment 2
[0019] a. Use the dried rhizome of yam crude drug as raw material, remove plant impurities, weigh 10 kg and pulverize, heat with water 5 times the weight of yam, temperature 100°C, reflux extraction 3 times for 3 hours, filter, and combine the extracts , filtered, concentrated to 50%;
[0020] b. Add 95% ethanol to the concentrated solution to make the alcohol content of the concentrated solution reach 30%, and remove the precipitate after precipitation occurs;
[0021] c. Continue to add 95% ethanol to the concentrated solution to make the alcohol content reach 90%. After the precipitate appears, let it stand for 6 hours. After the precipitate is fully precipitated, remove the supernatant to collect the precipitate, then dry it and crush it. , the yellow-brown powder yam polysaccharide content of 40-50% and the composition of yam protein content of 30-40% can be obtained.
Embodiment 3
[0023] a. Take the dried rhizome of Chinese yam as a raw material, remove plant impurities, weigh 10 kilograms and pulverize it, heat it with water 3.5 times the weight of Chinese yam, at a temperature of 80° C., reflux and extract 3 times for 2.5 hours, filter, and combine the extracts, Filter and concentrate to 50%;
[0024] b. Add 95% ethanol to the concentrated solution to make the alcohol content of the concentrated solution reach 30%, and remove the precipitate after precipitation occurs;
[0025] c. Continue to add 95% ethanol to the concentrated solution to make the alcohol content reach 90%. After the precipitate appears, let it stand for 4.5 hours. After the precipitate is fully precipitated, remove the supernatant to collect the precipitate, then dry it and crush it. , the yellow-brown powder yam polysaccharide content of 40-50% and the composition of yam protein content of 30-40% can be obtained.
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