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Application of d609 in the preparation of drugs for preventing and treating retinal damage diseases

A drug and omentum technology, applied in the field of biomedicine, to achieve the effect of reducing the degree of damage, strong protection, and reducing the level of release

Active Publication Date: 2020-11-13
广州因明生物医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports at home and abroad on the effect of D609 on RPE cells under oxidative stress. Whether D609 may delay the occurrence and development of AMD by reducing the oxidative stress damage of RPE cells remains to be studied.

Method used

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  • Application of d609 in the preparation of drugs for preventing and treating retinal damage diseases
  • Application of d609 in the preparation of drugs for preventing and treating retinal damage diseases
  • Application of d609 in the preparation of drugs for preventing and treating retinal damage diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Method

[0066] 1. Experimental materials and procedures

[0067] 1.1 Cell culture medium preparation: DMEM / F12+10% fetal bovine serum+1% P / S double antibody.

[0068] 1.2 Preparation of various antioxidants: Dilute different antioxidants separately, and use a concentration of 10 μg / mL.

[0069] 1.3 Preparation of sodium iodate: Sodium iodate (SI) was directly diluted with culture medium, and the final concentration was 10 mM.

[0070] 1.4 Treatment of cells:

[0071] The retinal pigment epithelial cell line ARPE-19 cells purchased from ATCC will be cultured in the medium of DMEM / F12+10% fetal bovine serum+1% double antibody, and divided into negative control group and model control group (sodium iodate) 1. Experimental group: Sodium iodate + various antioxidants were co-treated separately. Place at 37°C, 5% CO 2 Cultivated in an incubator. Observe cell changes and perform corresponding molecular biology tests.

[0072] (2) Results

[0073] The results are s...

Embodiment 2

[0081] (1) method

[0082] 1. Experimental materials and procedures

[0083] 1.1 Cell culture medium preparation: DMEM / F12+10% fetal bovine serum+1% P / S double antibody

[0084] 1.2 D609 preparation: Dilute D609 powder with ultrapure water, the final concentration is 5mg / mL, and the final use concentration is 10μg / mL.

[0085] 1.3 Preparation of sodium iodate: Sodium iodate (SI) was directly diluted with culture medium, and the final concentration was 10 mM.

[0086] 1.4 Treatment of cells:

[0087] The retinal pigment epithelial cell line ARPE-19 cells purchased from ATCC will be cultured in the medium of DMEM / F12+10% fetal bovine serum+1% double antibody, and divided into negative control group and model control group (sodium iodate) , Experimental group 1: sodium iodate + D609, experimental group 2: D609. Place at 37°C, 5% CO 2 Cultivated in an incubator. In the experimental group 1, D609 was added to the medium containing 10 mM sodium iodate and diluted to 10 μg / mL, ...

Embodiment 3

[0094] Example 3 Cell Viability Experiment (CCK8 Staining)

[0095] (1) Method

[0096] 1. Experimental materials and procedures

[0097] 1.1 Cell culture medium preparation: DMEM / F12+10% fetal bovine serum+1% P / S double antibody

[0098] 1.2 D609 preparation: Dilute D609 powder with ultrapure water, the final concentration is 5 mg / mL, and the final use concentration is 10 μg / mL.

[0099] 1.3 Sodium iodate preparation: The sodium iodate powder was directly diluted with culture medium, and the final concentration was 10mM.

[0100] 1.4 Cell preparation: ARPE-19 cells, a retinal pigment epithelial cell line purchased from ATCC, were seeded into 96-well plates, with 10,000 cells per well, and cultured for 48 hours. Divided into negative control group, model control group (sodium iodate), experimental group 1: sodium iodate + D609, experimental group 2: D609. In the experimental group 1, D609 was added to the medium containing 10 mM sodium iodate and diluted to 10 μg / mL, and t...

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Abstract

The invention discloses an application of D609 in preparation of a medicine for preventing and treating retinal injury diseases. The Chinese name of D609 is tricyclic decane-9-yl-dithiocarbonate potassium salt, and the D609 is an antiviral and antitumor small molecular compound, which belongs to a selective inhibitor of phosphatidylcholine specific phospholipase (PC-PLC). According to the invention, the wide and deep research is carried out, the unexpected discovery is realized for the first time, the expression of metallothionein is up-regulated by the D609; the oxidative damage of RPE cellscan be effectively inhibited; therefore, the compound can be used for preventing or treating retinal injury diseases caused by oxidative stress injury of retinal pigment epithelial cells, especially,the compound can be extremely effectively used for preventing or treating macular degeneration diseases of retina, has no obvious drug toxicity, can effectively control the occurrence and developmentof injury diseases of retina, and provides a new theoretical support for developing unknown biological activity of D609 and future clinical treatment effects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of tricyclic decanan-9-yl-dithiocarbonate potassium salt (D609) in the preparation of drugs for the prevention and treatment of retinal damage diseases, and opens up new possibilities for the prevention and clinical treatment of ophthalmology-related diseases. methods and ideas. Background technique [0002] The retinal pigment epithelium (RPE) is a layer of pigmented cells that adheres closely to the subretina. It is a neatly arranged single layer of hexagonal cells located between the optic nerve retina and Brunch's membrane. Maintaining the normal physiological function of RPE plays an important role in the normal reception and transmission of photoreceptor signals, mainly in: 1) regulating the transport of nutrients from choroidal capillaries to photoreceptor cells; 2) absorbing light, improving the quality of the visual system and reducin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/265A61K45/06A61P9/10A61P27/02
CPCA61K31/265A61K45/06A61P9/10A61P27/02A61K2300/00
Inventor 欧阳宏王力
Owner 广州因明生物医药科技股份有限公司
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