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OGP-AcAP5 fusion protein for treating osteoporosis and thrombosis and nucleic acid for coding same

A technology for osteoporosis and fusion protein, applied in the field of fusion protein and nucleic acid encoding the fusion protein, can solve the problems of patient inconvenience, antagonistic reaction, etc., and achieve the effect of safe use

Inactive Publication Date: 2014-09-10
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the patient takes multiple drugs to treat osteoporosis and thrombosis at the same time, drug antagonistic reactions are likely to occur; if the time of taking drugs is to be staggered, on the one hand, the half-life of the drug must be considered, and on the other hand, the effective concentration of the drug must also be considered, and it will cause inconvenience to the patient.
[0005] At present, there is no drug that can effectively treat osteoporosis and thrombosis at the same time

Method used

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  • OGP-AcAP5 fusion protein for treating osteoporosis and thrombosis and nucleic acid for coding same
  • OGP-AcAP5 fusion protein for treating osteoporosis and thrombosis and nucleic acid for coding same
  • OGP-AcAP5 fusion protein for treating osteoporosis and thrombosis and nucleic acid for coding same

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Experimental program
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specific Embodiment approach 1

[0012] Specific embodiment 1: The amino acid sequence of the OGP-AcAP5 fusion protein used for treating osteoporosis and thrombus in this embodiment is shown in SEQ ID NO:2.

[0013] The nucleic acid sequence of the OGP-AcAP5 fusion protein used in the treatment of osteoporosis and thrombosis in this embodiment is shown in SEQ ID NO: 1. The nucleic acid sequence was synthesized by a bioengineering company to make a plasmid vector pUC( OGP / AcAP5). The pUC (OGP / AcAP5) vector was double-digested with BamHI and EcoR I (the enzyme digestion system is shown in Table 1), and pGEX-6P-1 was double-digested with BamHI and EcoR I (the enzyme digestion system was shown in Table 1). 2); then the present embodiment is used for the connection of the DAN fragment (OGP / AcAP5) of the OGP-AcAP5 fusion protein (OGP / AcAP5) for the treatment of osteoporosis and thrombus with the carrier pGEX-6P-1 through double digestion (ligation reaction system as shown in Table 3).

[0014] Table 1

[0015] ...

specific Embodiment approach 2

[0058] Embodiment 2: The nucleic acid sequence of the OGP-AcAP5 fusion protein used in the treatment of osteoporosis and thrombus in this embodiment is shown in SEQ ID NO:1.

[0059] The nucleic acid sequence of the OGP-AcAP5 fusion protein used in the treatment of osteoporosis and thrombus in this embodiment was synthesized by a bioengineering company.

[0060] In this embodiment, the original signal peptide of the AcAP5 gene is removed from the nucleic acid sequence of the OGP-AcAP5 fusion protein used to treat osteoporosis and thrombosis, and a Kpn I restriction enzyme is inserted between the osteogenic growth peptide and the anticoagulant peptide of H. caninum The site can not only improve the stability of the encoded fusion protein, but also change the secondary structure of the fusion protein to improve its biological performance.

[0061] In this embodiment, BamHI and EcoR I restriction sites are respectively added on both sides of the fusion protein gene, and the codin...

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Abstract

The invention relates to an OGP-AcAP5 fusion protein for treating osteoporosis and thrombosis and a nucleic acid for coding the OGP-AcAP5 fusion protein, belonging to the fusion protein and the nucleic acid for coding the fusion protein, and aims to overcome the defect that no medicament for simutaneously treating osteoporosis and thrombosis is available at present. The OGP-AcAP5 fusion protein for treating osteoporosis and thrombosis has an amino acid sequence shown as SEQ ID NO:2, and the nucleic acid for coding the OGP-AcAP5 fusion protein has an amino acid sequence shown as SEQ ID NO:1. The OGP-AcAP5 fusion protein and the nucleic acid provided by the invention can be used in the pharmaceutical field.

Description

technical field [0001] The present invention relates to a fusion protein and nucleic acid encoding the fusion protein. Background technique [0002] The incidence of osteoporosis in the elderly is high. There are 200 million osteoporosis patients in the world, and there are more women than men. According to the standards of the World Health Organization (WHO), the results of the US National Health and Nutrition Survey (NHANES III, 1988-1994) showed that osteoporosis seriously affects the quality of life of the elderly. Among the people over 50 years old, 1 / 2 of women, One fifth of men will suffer from osteoporotic fractures in their lifetime. Once a patient experiences the first osteoporotic fracture, the risk of secondary fractures increases significantly. The elderly in China rank first in the world, and there are 90 million osteoporosis patients, accounting for 7.1% of the total population. With the aging of society, the incidence of osteoporosis is on the rise, and it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62A61P19/10A61P7/02
Inventor 余琼刘祺
Owner HEILONGJIANG UNIV