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Promoter from Litchi chinensis and application thereof

A lychee and DNA molecule technology, applied to cells modified by the introduction of foreign genetic material, angiosperms/flowering plants, etc., can solve problems such as differences in the temporal and spatial expression patterns of PPO genes

Active Publication Date: 2013-12-11
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It may be due to differences in promoter sequences that cause differences in the spatiotemporal expression patterns of PPO genes

Method used

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  • Promoter from Litchi chinensis and application thereof
  • Promoter from Litchi chinensis and application thereof
  • Promoter from Litchi chinensis and application thereof

Examples

Experimental program
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Embodiment 1

[0036] Embodiment 1, the acquisition of promoter derived from litchi

[0037] The main cause of rapid postharvest browning of litchi fruit may be the action of polyphenol oxidase (PPO). Previous studies also showed that the expression of litchi PPO gene was related to the browning of pericarp. The inventor of the present invention has discovered a new litchi PPO gene, cloned the partial promoter sequence of the litchi PPO gene, analyzed its partial cis-acting elements, and analyzed the differences in the PPO gene promoter sequences of different litchi varieties, The expression difference of GUS gene regulated by this promoter in litchi leaves, pericarp and seeds was studied.

[0038] One, the cloning of the PPO gene promoter of litchi pericarp

[0039] 1. Extraction of DNA from litchi peel:

[0040]The fruit of the eight-mature litchi variety "Concubine Xiao" was collected from the experimental field base of the Chinese Academy of Tropical Agricultural Sciences. Transport ...

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Abstract

The invention discloses a promoter and application thereof. The promoter has sequences of 1) a DNA sequence of a sequence 1 in the sequence table; 2) a DNA sequence of 82-1048th site from 5'terminal of the sequence 1 in the sequence table; 3) a DNA sequence of 379-1048th site from 5' terminal of the sequence 1 in the sequence table; 4) a DNA sequence of 622-1048th site from 5' terminal of the sequence 1 in the sequence table; 5) a DNA sequence having 90% of similarity with the DNA sequences defined as 1) 2) 3) or 4) and with promoter function; and 6) a nucleotide sequence capable of hybridizing with the DNA sequence defined by the sequence 1 in the sequence table under highly stringent conditions. The promoter provided by the invention can specifically express in pericarp and leave of Litchi chinensis, and can be used for cultivation of transgenic plants and control of tissue-specific expression of exogenous gene expression, and especially tissue specific expression in pericarp of Litchi chinensis.

Description

technical field [0001] The present invention relates to a promoter and its application in the biological field, in particular to a promoter derived from Litchi Feizixiao variety and its application. Background technique [0002] Litchi pericarp browning is generally designed in two aspects: one is non-enzymatic browning, and the other is enzymatic browning. Enzymatic browning is the main cause of litchi peel browning. Enzymatic browning means that phenolic substances in litchi are catalyzed by phenol oxidases to generate quinones; quinones can undergo self-polymerization in plants or react with amino acids and proteins in cells to produce black or brown substances, making litchi The peel is browned. At least three enzymes, anthocyanidase (ANT), peroxidase (POD) and polyphenol oxidase (PPO), were involved in the oxidation of phenols in the enzymatic browning of litchi peel. The peel contains a variety of phenolic substances, most of which can be oxidized by PPO and POD. C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12N5/10C12N1/21A01H5/00
Inventor 王家保张新春刘保华孙进华李焕苓
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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