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Semi-artificial cultivation method for amanita parvipantherina

A kind of semi-artificial and amanita technology, which is applied in the field of large-scale fungus cultivation and semi-artificial cultivation of wild poisonous fungus Amanita japonica, which can solve difficult problems and achieve the effects of low production cost, saving workshops and energy

Inactive Publication Date: 2013-04-03
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Facing the silent disappearance of these resources, we have not been paid attention to, and we have not actively taken various measures to save them, then this loss will be irreparable
[0006] The artificial domestication or cultivation of Amanita is the premise or key to ensure the sustainable utilization of Amanita resources, but it is still a problem and blind spot that is difficult to overcome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] Pick out the mycelium that grows well, remove the medium on it, and cut it into 4 to 6 parts, each part is 0.25cm 2 Gently embed it on the surface of a 500ml Erlenmeyer flask medium, the medium is a modified SPDM medium (potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B 1 0.10mg / L, agar 12.00g / L), supplemented wort juice (15.5 baume) 180ml, glutamic acid 0.20g / L, growth active substance 15mg / L, control the pH value of 5.4, culture temperature is 23 ℃, dark Cultivate for 15 days, and the hyphae will cover the triangle flask;

[0020] Inoculate the above well-growing mycelium into the cultivation medium (50% crushed original mulch + 50% bran) of a 1000ml Erlenmeyer flask, add 150ml of wort juice (16.5 Baume) and 0.10 glutamic acid g / L, growth active substance 10mg / L, adjust PH value to 5.4, culture temperature is 23°C, culture in dark for 28 days, the hyphae basically cover the ...

example 2

[0024] Pick out the mycelium that grows well, remove the medium on it, and cut it into 4 to 6 parts, each part is 0.25cm 2 Gently embed it on the surface of a 500ml Erlenmeyer flask medium, the medium is a modified SPDM medium (potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B 1 0.10mg / L, agar 12.00g / L), supplemented wort juice (15.5 baume) 180ml, glutamic acid 0.20g / L, growth active substance 15mg / L, control the pH value of 5.4, culture temperature is 23 ℃, dark Cultivate for 15 days, and the hyphae will cover the triangle flask;

[0025] Inoculate the above well-grown mycelium into the cultivation medium (50% crushed in situ humus + 50% bran) of a 1000ml Erlenmeyer flask, supplemented with 180ml of wort (15.5 baume) and 0.25 glutamic acid g / L, growth active substance 15mg / L, adjust PH value to 5.5, culture temperature is 24°C, culture in dark for 30 days, mycelia basically cover th...

example 3

[0029] Pick out the mycelium that grows well, remove the medium on it, and cut it into 4 to 6 parts, each part is 0.25cm 2 Gently embed it on the surface of a 500ml Erlenmeyer flask medium, the medium is a modified SPDM medium (potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B 1 0.10mg / L, agar 12.00g / L), supplemented wort juice (15.5 baume) 180ml, glutamic acid 0.20g / L, growth active substance 15mg / L, control the pH value of 5.4, culture temperature is 23 ℃, dark Cultivate for 15 days, and the hyphae will cover the triangle flask;

[0030] Inoculate the above well-growing mycelium into the cultivation medium (50% crushed original mulch + 50% bran) of a 1000ml Erlenmeyer flask, add 160ml of wort juice (15.0 Baume) and 0.20 glutamic acid g / L, growth active substance 13mg / L, adjust PH value to 5.6, culture temperature is 22, dark culture for 25 days, mycelia basically covered the triang...

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PUM

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Abstract

The invention relates to a semi-artificial cultivation method for amanita parvipantherina, belonging to the technical field of large fungus culture. The semi-artificial cultivation method is characterized in that a large number of hyphae which are obtained in a laboratory through solid culture are directly inoculated into an outdoor natural habitat; and the hyphae are grown and developed by adding a growth promotion substance to finally grow fruiting bodies. Mycelia are directly induced to fruit by using natural growth conditions; and the method has the advantages of easiness in operation, original ecology, no pollution, energy saving, capability of saving a factory building and the like, and is a more ideal culture mode at present. The amanita is a special and precious large economic fungus, has high value and a wide application range, has potential application prospect in the fields of developing new specific medicines such as anti-tumor medicines, anti-bacterial and anti-viral medicines, sedatives or narcotic and the like, is difficult to develop and apply due to the problems such as valuable and rare resource, difficulty in artificial domestication and inactivity of chemical synthetic products of toxin up to now, is used as a peptide toxin of a biochemical reagent and is worth over one hundred thousand dollars every gram. The fact that the hyphae are difficultly developed into the fruiting bodies under the culture conditions is a key factor for restricting the artificial culture; and taking the amanita parvipantherina for instance, certain breakthrough is realized aiming at the problem, so that the artificial culture of the parvipantherina becomes possible, and conditions are created for sustainable development and utilization of the resource.

Description

technical field [0001] The invention relates to a method for semi-artificial cultivation of wild poisonous fungus Amanita japonica, which belongs to the field of biotechnology, and specifically belongs to the category of large fungus cultivation. Background technique [0002] Amanita ( Amanita ) belongs to Basidiomycotina, Phytomycetes, Agaricales, Amanitaceae ( Amanitaceae ), is a more special and valuable worldwide widespread genus among poisonous macrofungi, and its species diversity is very rich. Nearly 400 species have been reported in the world, and nearly 100 species (including subspecies, variants and variants) have been recorded in China. According to research, there are still many species in China that have not yet been studied and named. However, with the emergence of the global ecological crisis, many large fungal resources in our country are in danger, in a serious decline and endangered state, and some species are facing the risk of extinction before they a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 李宗菊张曦予刘弟
Owner YUNNAN UNIV
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