Wide-frequency-band optical-acoustic and fluorescent double-imaging device without energy converter and detection method thereof

Abstract

The invention discloses a wide-frequency-band optical-acoustic and fluorescent double-imaging device without an energy converter and a detection method thereof. The device comprises an optical-acoustic / fluorescent stimulating light source, an optical-acoustic signal detection light source assembly, an optical-acoustic signal detection assembly, a fluorescent signal detection assembly and an optical-acoustic / fluorescent signal collecting / processing assembly, wherein the optical-acoustic / fluorescent stimulating light source and the optical-acoustic signal detection light source assembly are respectively connected with the optical-acoustic signal detection assembly and the optical-acoustic / fluorescent signal collecting / processing assembly; the optical-acoustic detection assembly is respectively connected with the fluorescent signal detection assembly and the optical-acoustic / fluorescent signal collecting / processing assembly; and the fluorescent signal detection assembly is connected with the optical-acoustic / fluorescent signal collecting / processing assembly. According to the wide-frequency-band optical-acoustic and fluorescent double-imaging device without the energy converter and the detection method thereof, an optical-acoustic signal is produced by using pulse laser to irradiate a biological sample; continuous laser with a narrower line width is focused on the surface of a sample and the aim of detecting an optical-acoustic signal is achieved by detecting the vibration speed of the surface of the sample; and a bandwidth limitation detect of the energy converter is eliminated and the organic combination of optical-acoustic and fluorescent double-imaging is realized.

Description

technical field [0001] The invention belongs to the technical field of photoacoustic imaging, and in particular relates to a broadband transducer-less photoacoustic and fluorescent dual imaging device and a detection method thereof. Background technique [0002] When a certain absorber is irradiated with light, the absorber absorbs the light energy and produces a temperature rise, and the temperature rise and fall causes the volume of the absorber to expand and shrink, and ultrasonic waves are generated. This phenomenon is called the photoacoustic effect. The photoacoustic effect has attracted people's attention since it was discovered in the 19th century, and it has been applied in various fields to varying degrees. As a new imaging technology, photoacoustic imaging has been applied in more and more fields. This imaging technology uses short-pulse laser as the excitation source, and the ultrasonic signal excited by it as the information carrier. By reconstructing the image...

AI Technical Summary

Problems solved by technology

[0003] Traditional photoacoustic signal detection tools are generally ultrasonic transducers, and photoacoustic signals have a wide frequency band, but traditional ultrasonic transducers are limited by materials, and generally have narrow frequency bands

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