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Cell culture pipeline device and automatic cell culture system

Active Publication Date: 2013-04-10
浙江赛尚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires experienced and well-trained staff, and this labor-intensive training method is not efficient
The second method is to use disposable blood bags or hollow fiber culture tubes. This culture method has the problems of complicated culture operation and low culture efficiency.

Method used

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  • Cell culture pipeline device and automatic cell culture system
  • Cell culture pipeline device and automatic cell culture system
  • Cell culture pipeline device and automatic cell culture system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Such as figure 1 As shown, a cell culture pipeline device includes a main circulation pump 20, a gas exchange tube 21, a culture tube 23, and a culture fluid main reservoir 19 that are sequentially connected by a flow guide tube, and a culture fluid main reservoir 19 is provided A micro-aperture exhaust hole 191 communicated with the atmosphere. The gas exchange tube 21 is a vulcanized silicone tube, and the main circulation pump 20 is a finger peristaltic pump. The culture tube 23 is a hollow fiber culture tube. The draft tube includes a first draft tube 11 connecting the main circulation pump 20 and the gas exchange tube 21, a second draft tube 12 connecting the gas exchange tube 21 and the culture tube 23, and connecting The culture tube 23 and the third guide tube 13 of the culture solution main reservoir 19 and the fourth guide tube 14 connecting the culture solution main reservoir 19 and the main circulation pump 20 are transparent in this embodiment The test con...

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PUM

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Abstract

The invention discloses a cell culture pipeline device, which comprises a main circulating pump, a gas exchange tube, a culture tube and a culture solution main library which are sequentially connected through a guide tube, wherein a micro-pore exhaust hole communicated with the atmosphere is formed on the culture solution main library; the gas exchange tube is a cured silicone tube; and the main circulating pump is a finger peristaltic pump. The invention also discloses an automatic cell culture system, which comprises a sensor arranged nearby a transparent testing container, and also comprises a liquid filling device which is connected with a first guide tube through a fifth guide tube and a waste liquid recovery device which is connected with a third guide tube through a sixth guide tube. The cell culture pipeline device is simple in structure, easy and convenient to operate and high in culture efficiency, as well as the automatic cell culture system employing the cell culture pipeline device.

Description

Technical field [0001] The invention relates to a cell culture device and system, in particular to a cell culture pipeline device and a cell automated culture system. Background technique [0002] T cells are important white blood cells that help the immune response against cancer and viral infections. They are produced in the thymus and have a long-term immune memory to prepare for the need for rapid response in the immune response. Once activated by an antigen, T cells can replicate rapidly and produce a rapid antigen response. In some chronic viral infections and cancers, T cells are inactivated or function insufficiently. In the case of viral infection, the immune response cannot activate T cells and kill the infected cells, which can lead to chronic infection. Chronic infection can lead to long-term disabling diseases (such as chronic active hepatitis) and even death (such as AIDS). Chronic infection, as well as many other factors, can also cause genetic mutations in inf...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36
Inventor 荆杰
Owner 浙江赛尚医药科技有限公司
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