A preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same

A chemiluminescence and chemiluminescence reagent technology, applied in the construction of a microfluidic paper chip laboratory technology platform, can solve the problems of low sensitivity, poor selectivity, and false positive results of colorimetric methods, so as to improve the sensitivity and reduce the preparation cost. , the effect of simplifying the detection steps

Inactive Publication Date: 2013-04-24
UNIV OF JINAN
View PDF4 Cites 44 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the analysis method based on the microfluidic paper chip technology platform is mainly the colorimetric method, because the colorimetric method can only give a "yes / no" signal response, and the colorimetric method has low sensitivity and poor selectivity , prone to false positive results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same
  • A preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same
  • A preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 On-site rapid simultaneous detection of glucose, polyphenols, xanthines, cholesterol, uric acid, and heme in human blood.

[0057] (1) Use Adobe Illustrator CS4 software on the computer to design the hydrophobic wax batch printing pattern of the microfluidic chemiluminescence paper chip (the style is as attached figure 1 shown). The dimensions are: central sample injection area (10.0 mm in diameter), six surrounding chemiluminescent detection areas (4.0 mm in diameter) and six enzyme-recognizing catalytic microchannels (both width 2.0 mm, six enzyme-recognizing catalytic microchannels The decibels of the channels are 5.0 mm; 8.0 mm; 11.0 mm; 14.0 mm; 17.0 mm; 20.0 mm. The size of the microfluidic chemiluminescent paper chip is 50.0 mm × 50.0 mm. At this time, one A4 paper can print 15 microfluidic Controlled chemiluminescent paper chips.

[0058] (2) Use Adobe Illustrator CS4 software on the computer to design batch printing patterns of chemiluminescent re...

Embodiment 2

[0071] Example 2 On-site rapid simultaneous detection of glucose, polyphenols, xanthines, cholesterol, uric acid, and heme in human urine.

[0072] "Utilize Adobe Illustrator CS4 software design" in embodiment 1 step (1) (2) (3) to " utilize Photoshop CS4 software design "; Change " qualitative filter paper " in embodiment 1 into " quantitative filter paper " ; Change Example 1 step (1) to: "Utilize Photoshop CS4 software to design the hydrophobic wax batch printing pattern of microfluidic chemiluminescent paper chip on the computer (style as attached figure 1shown). The dimensions are: central sample injection area (7.5 mm in diameter), six surrounding chemiluminescence detection areas (3.0 mm in diameter) and six enzyme recognition catalytic microchannels (1.5 mm in width each, six enzyme recognition catalytic microchannels The decibels of the channels are 3.75 mm; 6.0 mm; 8.25 mm; 10.5 mm; 12.75 mm; 15.0 mm. The size of the microfluidic chemiluminescent paper chip is 37....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The present invention discloses a preparation method of a simple, low-cost, multi-channel microfluidic chemiluminescent paper chip and an on-site detection method by using the same. By using a full print mode, a plurality of multi-channel microfluidic chemiluminescent paper chips are printed out on a common A4-size filter paper in batch. The printing process includes: printing hydrophobic wax patterns in batch; melting wax for molding; printing chemiluminescent reagent ink patterns in batch; printing oxidase ink pattern in batch; cutting the microfluidic chemiluminescent paper chips into certain shape; and performing plastic package to the prepared microfluidic chemiluminescent paper chips. An on-site detection method by using the microfluidic chemiluminescent paper chip comprises the steps of: placing the plastic packaged microfluidic chemiluminescent paper chip into the cartridge of a handheld luminescent detector; dropwise adding a sample solution into the sample region; then covering the cartridge with a lid, and starting the test. The existence and contents of glucose, polyphenols, xanthine, cholesterol, uric acid, and hemoglobin are sequentially determined via the size of six chemiluminescence peaks sequentially appearing.

Description

[0001] technical field [0002] The invention relates to the technical field of on-site instant analysis and detection with low cost, high throughput and simple operation, and more specifically relates to the construction of a microfluidic paper-on-a-chip laboratory technology platform suitable for chemiluminescent enzyme analysis. Background technique [0003] At present, there are mainly two ways of in vitro (clinical) diagnosis: one is to use the fully automated and highly sensitive large-scale instruments and equipment in the central analysis laboratory of the hospital to achieve high-precision disease analysis and diagnosis; the other is the diametrically opposite way It realizes on-site rapid analysis and diagnosis through small and simple handheld devices. Due to the aging of the population, the incidence of adult diseases has increased sharply. If relying solely on the central laboratory of the hospital, the health cost of the people will be greatly increased. There...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/76
Inventor 于京华葛磊王寿梅颜梅葛慎光王衍虎王盼盼张彦李蒙刘伟艳苏敏孙国强闫纪宪臧德进王少伟
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap