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Preparation method for bacteria liquid beneficial for growth-development and mycorrhiza formation of rhododendron plants

A Rhododendron, plant growth technology, applied in the directions of botanical equipment and methods, microorganism-based methods, plant growth regulators, etc. rate effect

Active Publication Date: 2013-04-24
TONGHUA NORMAL UNIV
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  • Claims
  • Application Information

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Problems solved by technology

Domestic research on rhododendron mycorrhizal fungi is in its infancy, and there are few related reports, most of which are in the basic research stage and have not entered the application field.

Method used

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Embodiment Construction

[0019] A method for preparing a bacterial liquid that is beneficial to the growth and development of Rhododendron plants and the formation of mycorrhizae, the steps are as follows:

[0020] 1 Materials and handling

[0021] Collecting Rhododendron oxhide in Changbai Mountains ( Rhododendron chrysanthum Pall.), short-fruited cuckoo ( Rhododendron brachycarpum D. Don), Zhaobai Rhododendron ( Rhododendron micranthum Turcz.), Rhododendron microphylla ( Rhododendron parvifolium Adams.), Rhododendron ( Rhododendron schlippenbachii Maxim.), Xing'an Rhododendron ( Rhododendron dauricum L.), Red Rhododendron ( Rhododendron mucronulatum Turcz.), felt cuckoo ( Rhododendron confertissimum Nakai) and rhododendron ( Rhododendron redowskianum Maxim.) and other 9 species of Rhododendron slender roots. After breaking the soil, cut mature fibrous roots with a diameter of less than 1.0 mm near the thick roots, gently wash off the soil with a soft brush, rinse with runnin...

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Abstract

The invention relates to a bacteria liquid for growth and development of plants, and in particular relates to a preparation method for bacteria liquid beneficial for growth-development and mycorrhiza formation of rhododendron plants. The method comprises the following steps: (1) preparation and treatment of materials: collecting and disinfecting mature fibrous roots of rhododendron plants, and cutting the treated mature fibrous roots to root segments for later use; (2) culture medium configuration: the content of 1000mL of culture medium comprises the following components of 380mg of KH2PO4, 149mg of MgSO4.7H2O, 26mg of vitamin C, 67mg of calcium pantothenate, 1.4mg of nicotinic acid, 3.0g of peptone, 16.5g of glucose, 8.5g of agar powder, 4mL of 1.5% of sodium deoxycholate liquid and 0.05mg of indoleacetic acid; (3) preparation of mother liquid; and (4) preparation of the bacteria liquid. The preparation method provided by the invention provides a technical method for production, application and fundamental research on introduction, artificial breeding, cultivation, breeding and the like of the rhododendron plants in Changbai Mountain and other rhododendron plants at home and abroad.

Description

[0001] technical field [0002] The invention relates to a plant growth and development bacterial liquid, that is, a preparation method of the bacterial liquid beneficial to the growth and development of Rhododendron plants and the formation of mycorrhizae. Background technique [0003] In the prior art, Rhododendron is a world-renowned ornamental plant and also one of my country's top ten traditional famous flowers. Rhododendron resources are very rich in my country, and it is an important resource and geographical distribution center of Rhododendron. However, research on the introduction, artificial breeding, cultivation and breeding of wild rhododendrons at home and abroad is slow. The reason is mostly due to the difficulties in artificial propagation, cultivation, growth and development of rhododendrons, especially wild alpine rhododendrons. . Rhododendrons in Changbai Mountains include Rhododendron Rhododendron chrysanthum Pall.), short-fruited cuckoo ( Rhododendron ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 顾地周顾川岳朱俊义姜云天陆爽潘雨禚玲玲张学士王秋爽付航
Owner TONGHUA NORMAL UNIV
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