Primer for weedy rice detection and application of primer

Abstract

The invention discloses a primer for weedy rice detection as well as a molecular detection reagent kit and a detection method adopting the primer. The primer for detection comprises a positive primer 5'-TTACAGGGGAGCAGAAACA-3' and a reverse primer 5'-TCTTCTCCTCTCTTTCAGCAC-3'. According to the detection method, after the DNA (deoxyribonucleic acid) extraction, the primer is used for PCR (polymerase chain reaction) amplification, electrophoretic separation is carried out, and colouration observation is carried out. When the detection method provided by the invention is utilized, the weedy rice, the culture rice and the field hybrid rice produced between the weedy rice and the culture rice can be effectively detected.

Description

technical field [0001] The invention relates to a primer for rapid detection of weedy rice. Background technique [0002] Weedy rice is often associated with cultivated rice and is widely distributed, almost existing in all rice production areas in the world. In some rice-growing countries in the temperate zone of the world, the damage has become the third largest weed after barnyardgrass and chinensis, with a density of 2-40 plants / m 2 The rice yield can be reduced by 19%-89% in one hour, and the rice yield can be reduced by 61% in the whole season, and the spreading speed is accelerating, and the degree of damage is constantly escalating. [0003] The reason why weedy rice will cause greater harm to the production of rice is mainly due to: 1) its similarity with cultivated rice makes it consistent with the response of cultivated rice to herbicides, and there is no effective herbicide in the world 2) Weeds have strong characteristics, mainly manifested in seed sha...

AI Technical Summary

Problems solved by technology

[0005] Currently, there are no effective herbicides in the world due to the similarity of weedy rice to cultivated rice, which makes it respond to herbicides in the same way as cultivated rice

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