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Establishment of the tissue culture system of Populus ash

A method for establishing a poplar ash group, a technique for the establishment of a poplar ash group, which can be used in the field of establishing a tissue culture system of ash poplar, which can solve the problems that have not yet been seen in the research literature on the genetic transformation and regeneration system of ash poplar

Inactive Publication Date: 2016-03-16
SHAANXI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under natural conditions, previous studies reported that the population range of Populus cinerea was expanded mainly through seed and root tiller propagation, but so far, no research literature on the genetic transformation and regeneration system of Populus cinerea has been seen.

Method used

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  • Establishment of the tissue culture system of Populus ash
  • Establishment of the tissue culture system of Populus ash
  • Establishment of the tissue culture system of Populus ash

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Select robust Populus gray plants, cut the leaves of Populus gray poplar perpendicular to the main vein, inoculate them on various dedifferentiation mediums, explore the best formula (see Table 1), culture in darkroom, subculture every 20 days once.

[0014] Table 1 Medium used for populus gray callus induction (mg / L)

[0015]

[0016] The callus induced by Populus gray on No. 4-9 medium is light brown, water-soaked, slow growth, poor regeneration ability, and easy to die; the callus induced on No. 1, 2, and 3 medium is light yellow , fast growth, loose texture, the callus induced on the No. 3 medium began to brown after 25-28 days of culture, but the No. 1 and No. 2 callus browned faster and stopped growing than No. 3, and after 18 days of culture It starts to brown. So MS+Kinetin0.1mg / L+NAA0.3mg / L+2,4-D0.5mg / L is the best culture medium for Populus cinerea.

[0017] (2) Take about 3 g of loose and vigorously growing callus tissue, transfer it to about 50 mL ...

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Abstract

The invention discloses a method for establishing a tissue culture system of gray poplar. According to the method, gray poplar stem is adopted as explant material; and basis is provided for the factory production and the industrial production of the gray poplar through the steps of root induction, transplantation and the like. Through the method disclosed by the invention, the purposes of clone establishment, rapid propagation and factory seedling are achieved.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for establishing a poplar tissue culture system. Background technique [0002] Ash poplar (Populus×canescens=Populustremula×P.alba) is a plant of the genus Populus in the family Salicales, and is the main tree species in the desert riparian forest. Under natural conditions, previous researches reported that the population range of Populus cinerea was expanded through seed and root tiller propagation, but so far, there has been no research literature on the genetic transformation and regeneration system of Populus cinerea. Contents of the invention [0003] The object of the present invention is to provide a method for establishing populus ash tissue culture system, in order to realize the object of the present invention, it is proposed to adopt the following technical scheme: [0004] The present invention relates to the establishment method of Populus ash tissue cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 代惠萍赵桦霍科科薛小荣贾根良魏安智
Owner SHAANXI UNIV OF TECH
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