A cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cell source and its preparation method and application

A technology of pluripotent stem cells and cardiomyocytes, applied in the field of biomaterials, can solve the problems of poor adhesion of cell patches, single function, limited cell source, etc., to avoid ethical problems, ensure directionality, and have the effect of strong functionality

Active Publication Date: 2020-12-22
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a cardiomyocyte conductive microneedle patch integrated with an induced pluripotent stem cell source and its preparation method and application to solve the problems of poor adhesion, single function, limited cell source, and uncontrollable cell orientation in traditional cell patches. Disadvantages of difficulty in simulating the activity of real heart tissue

Method used

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  • A cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cell source and its preparation method and application
  • A cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cell source and its preparation method and application
  • A cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cell source and its preparation method and application

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Embodiment 1

[0030] This embodiment provides a cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cells, which consists of successively stacked microneedle array layers, parallel carbon nanotube layers integrated with induced pluripotent stem cell-derived cardiomyocytes, and composed of hydrogel scaffold layers.

[0031] The preparation method of the cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cells comprises the following steps:

[0032] Step 1, preparation of methacrylylated hyaluronic acid conductive microneedle patch.

[0033] Preparation of methacrylylated hyaluronic acid (5%, w / v) prepolymer solution containing 1% (v / v) photoinitiator (2-hydroxy-2-methylpropiophenone), i.e. hydrogel For the prepolymer solution, add the solution dropwise into the microneedle template of PDMS, and ultrasonically treat it for 10 minutes to remove all air bubbles so that the hydrogel prepolymer solution completely penetrates all th...

Embodiment 2

[0040] This embodiment provides a cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cells, which consists of successively stacked microneedle array layers, parallel carbon nanotube layers integrated with induced pluripotent stem cell-derived cardiomyocytes, and composed of hydrogel scaffold layers.

[0041] The preparation method of the cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cells comprises the following steps:

[0042] Step 1, preparation of methacrylylated gelatin conductive microneedle patch.

[0043] A solution of methacrylated gelatin (15%, w / v) prepolymer containing 1% (v / v) photoinitiator (2-hydroxy-2-methylpropiophenone) was prepared and doped with BSA- FITC to its final concentration of 0.5 mg / mL and 1 mg / mL, that is, to prepare a hydrogel prepolymer solution dissolved with therapeutic drugs. Two prepared hydrogel prepolymer solutions with different BSA-FITC concentrations were dropped in...

Embodiment 3

[0055] This embodiment provides a cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cells, which consists of successively stacked microneedle array layers, parallel carbon nanotube layers integrated with induced pluripotent stem cell-derived cardiomyocytes, and composed of hydrogel scaffold layers.

[0056] The preparation method of the cardiomyocyte conductive microneedle patch integrated with induced pluripotent stem cells comprises the following steps:

[0057] Step 1, preparation of methacrylylated gelatin conductive microneedle patch.

[0058] Preparation of methacrylylated gelatin (15%, w / v) prepolymer solution containing 1% (v / v) photoinitiator (2-hydroxy-2-methylpropiophenone) and spiked with vascular endothelialization The factor VEGF (1 μg / mL) and the anti-inflammatory factor IL-10 (1 μg / mL) are made into a hydrogel prepolymer solution dissolved with therapeutic drugs. The prepared hydrogel prepolymer solution was dropped into the ...

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Abstract

The invention discloses a conductive micro-needle patch integrated with myocardial cells derived from induced pluripotent stem cells and a preparation method for the conductive micro-needle patch andapplication of the conductive micro-needle patch. The patch consists of a micro-needle array layer, carbon nanotube layers and a hydrogel scaffold layer which are sequentially superimposed, wherein the carbon nanotube layers are integrated with the myocardial cells derived from the induced pluripotent stem cells and are arranged in parallel. A hydrogel prepolymer solution fills pores of a templateand is solidified to form the micro-needle array layer, then the carbon nanotubes layers arranged in parallel are deposited, and finally, the hydrogel prepolymer solution is filled again and is solidified, and the conductive micro-needle patch is obtained after the template is stripped; and the conductive micro-needle patch is used for subsequent differentiation culture of the induced pluripotentstem cells. The patch provided by the invention has a simple preparation method, is not restricted by cell sources, and is expected to become an effective therapeutic carrier for treatment of myocardial infarction.

Description

technical field [0001] The invention belongs to the field of biological materials and relates to a patch material, in particular to a cardiomyocyte conductive microneedle patch integrated with an induced pluripotent stem cell source and a preparation method and application thereof. Background technique [0002] The heart provides a steady stream of power for the human circulatory system, supplies oxygen and nutrients to tissues and organs through blood transport, and takes away metabolic waste, which is of great significance for maintaining basic human life activities. In recent years, the incidence of various cardiovascular diseases, especially myocardial infarction, has continued to increase, seriously threatening human health, and has become a thorny social problem. Therefore, it is one of the urgent problems to be solved in the field of biomedicine to develop an effective treatment method capable of reconstructing the function of infarcted myocardial tissue. [0003] Cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/08A61L27/22A61L27/20A61L27/50A61L27/54A61M31/00A61N1/05
CPCA61L27/08A61L27/20A61L27/222A61L27/227A61L27/3834A61L27/3839A61L27/3895A61L27/50A61L27/54A61L2300/602A61L2400/12A61L2430/20A61M31/002A61N1/059C08L5/08C08L5/04C08L89/00
Inventor 赵远锦孙灵钰王月桐
Owner NANJING DRUM TOWER HOSPITAL
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