Separation and culturing method of saussurea involucrate protoplast

Abstract

The invention relates to a separation and culturing method of Sinkiang saussurea protoplast, comprising four steps of culturing of sterile saussurea involucrate seedlings, inducement and differentiation of embryonic callus, separation and purification of plasmogen and culturing of the protoplast which are all applicable to the separation and culturing of the Sinkiang saussurea involucrate protoplast. The invention has simple and easily-operated method and lower requirements on the equipment and culturing conditions; in addition, the enzymolysis method is practical, the conditions for cell wall removal are mild, and the separated protoplast has high yield and strong activity, and is easy for the cultuing of the saussurea involucrate protoplast and a plant regeneration, thus laying the foundations for the culturing and fusing of the protoplast as well as the germplasm resource innovation of the Sinkiang saussurea involucrate and the research of transgenic saussurea involucrate.

Description

Isolation and cultivation method of snow lotus protoplast technical field The invention relates to the establishment of a suspension cell line of Saussurea sauraceae, in particular to a method for isolating and culturing protoplasts of Saussurea sauvignon. Background technique Snow lotus, (SaussureainvolucrataKar.etKir.etMaxim) belongs to Compositae and is a national second-class endangered plant. It is a perennial herb with a height of 15-25 (-35) cm; the rhizome is thick, dark brown, and the base is tan; the stem is single Healthy, erect, hollow, 2-4 cm in diameter, glabrous, dense leaves, subleathery, green, oblong or ovate-oblong, about 14 cm long, 2-4 cm wide, apex blunt or slightly Acute, base decelerates, margin has sparse small serrations, papillary glandular hairs, uppermost bracts 13-17, membranous transparent, light yellow, margins with neat sparse teeth, slightly glandular hairs, apex obtuse, base contracted , often exceeding inflorescence 2-fold. Capitulum 1...

AI Technical Summary

Problems solved by technology

Therefore, it is a feasible and effective way to use the protoplast culture method to innovate the germplasm resources of Xinjiang Saussurea sauraceae.

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