Method for inducing masculinization of fugu rubripes at low temperature

A red-finned pufferfish, low temperature induced technology, applied in the direction of climate change adaptation, fish farming, application, etc., to achieve the effect of being easy to operate, conducive to large-scale production and use, and avoiding operating procedures

Active Publication Date: 2013-07-24
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report on the masculin

Method used

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  • Method for inducing masculinization of fugu rubripes at low temperature
  • Method for inducing masculinization of fugu rubripes at low temperature
  • Method for inducing masculinization of fugu rubripes at low temperature

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0012] Example 1:

[0013] Select healthy male and female redfin puffer fish with high plumpness and well-developed gonads for artificial insemination. Insemination eggs are incubated at normal water temperature of 20℃ for 50 days, and then placed at 16℃ for 50 days. Other conditions for incubation and cultivation Same as existing technology.

[0014] At the same time, normal water temperature (20°C) was used to incubate and cultivate fertilized eggs for 100 days according to the method of the prior art as a control group.

[0015] Gender identification: 33 seedlings were taken from the control group and 40 seedlings from Example 1 of the present invention. The gonads were removed, fixed with Bouin's solution, and replaced with 80% alcohol after 6 hours. Use conventional alcohol gradient dehydration, transparent xylene, paraffin embedding, and continuous sectioning with a LEICA-RM 2016 microtome, with a thickness of 7 μm. It was stained with hematoxylin-eosin (H.E), mounted with ne...

Example Embodiment

[0019] Example 2:

[0020] Healthy male and female redfin puffer fish with high plumpness and well-developed gonads were selected for artificial insemination. The insemination eggs were incubated at 20°C for 75 days, and then placed at 15°C for 35 days.

[0021] The proportion of males in Example 2 of the present invention can reach 80%.

Example Embodiment

[0022] Example 3:

[0023] Select healthy male and female redfin puffer fish with high plumpness and well-developed gonads for artificial insemination. Insemination eggs are incubated at 20℃ for 45 days, and then placed at 17℃ for 65 days.

[0024] The proportion of males in Example 3 of the present invention can reach 65%.

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Abstract

The invention discloses a method for inducing masculinization of fugu rubripes at a low temperature. The method comprises the following steps of: performing artificial induced spawning and insemination on healthy male and female fishes which are high in fatness and undergo good gonad development; hatching fertilized eggs under the water temperature condition of 20 DEG C for 45-75 days; and putting into water of 15-17 DEG C, and culturing for 35-65 days. Due to the adoption of the method, male fugu rubripes fries can be produced rapidly, the male rate in the fries is 65-80 percent, the average male rate is 75 percent, ultraviolet ray irradiation is not required, sex hormone is not fed, and complex operation processes are avoided; and the method is low in cost, is easy to operate, and has contribution to mass production.

Description

technical field [0001] The invention relates to a method for raising seedlings of red-finned puffer, in particular to a method for inducing masculinization of red-finned puffer at low temperature without ultraviolet radiation, without feeding sex hormones and with simple operation. Background technique [0002] Redfin Pufferfish ( Takifugu rubripes ) is the rare fish with the largest individual, the least toxicity and the highest economic value in the genus Puffer. Because of its delicious meat, rich in protein, low in fat and rich in vitamins B1, B2, selenium, zinc and other trace elements, it has always been favored by Japan, South Korea and Chinese, especially the Japanese will be red fin Oriental Pufferfish is regarded as the "king of fish", and pufferfish cuisine - "sashimi" is the leading dish. In addition, the Japanese transliteration name "Fugu" has auspicious meaning, so it has always been an important representative of Japanese food culture. [0003] Studies have...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李雅娟姜志强杨凤李佳奇王玉生
Owner DALIAN OCEAN UNIV
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