Method of producing pearls on the basis of pearl shell mantel cell culture

A mantle cell and pearl oyster technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of difficult recovery, low pearl production rate, high mortality rate, improve survival rate and pearl yield rate, and reduce damage degree, the effect of reducing the number of

Active Publication Date: 2013-09-25
BEIHAI XIUPAI JEWELRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this method needs to consume a large amount of pearl oysters to provide small pieces of mantle, and at the same time, it will cause great damage to the pearl oysters a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) The choice of pearl oyster and yuzhu oyster: choose the pearl oyster as the Hyriopsis mussel, intact and 1 year old, and the yuzhu oyster as the Hyriopsis mussel, also intact and 1 year old.

[0014] (2) Sectioning: tear off the mantle from the pearl oyster and place it in the mixed solution containing penicillin streptomycin for 40 minutes. The concentration of penicillin in the mixed solution is 2000U / mL and the concentration of streptomycin is 2500U / mL. Then rinse with Hanks solution 3 times to remove blood stains, and then cut into 2mm×3mm pieces.

[0015] (3) Cell culture: Add the small pieces obtained in step (2) to the enzyme solution with a concentration of 60 units / mL for enzymatic digestion. The enzyme used is collagenase. Incubate at 37°C for 12 hours, centrifuge the digestion solution at 800 rpm for 6 minutes, and discard The supernatant was used to obtain pearl oyster mantle cells, and then the culture medium containing serum was added to the obtained cells,...

Embodiment 2

[0018] (1) The choice of pearl oyster and Yuzhu shellfish: choose the pearl oyster as the white butterfly shell, which is intact, and the age is 1 year old, and the Yuzhu shell is the white butterfly shell, which is also intact and the age is 2 years old.

[0019] (2) Sectioning: tear off the mantle from the pearl oyster, and place it in the mixed solution containing penicillin streptomycin for 60 minutes. The concentration of penicillin in the mixed solution is 2000 U / mL and the concentration of streptomycin is 2500 U / mL. Then rinse with Hanks solution 4 times to remove blood stains, and then cut into 2mm×3mm pieces.

[0020] (3) Cell culture: Add the small pieces obtained in step (2) to the enzyme solution with a concentration of 80 units / mL for enzymolysis and digestion. The enzyme used is trypsin. Incubate at 37°C for 15 hours, centrifuge the digestion solution at 800 rpm for 6 minutes, and discard The supernatant was used to obtain pearl oyster mantle cells, and then the cultu...

Embodiment 3

[0024] (1) The choice of pearl oyster and pearl oyster: choose the pearl oyster as the penguin oyster, which is intact, and the age is 0.5 years old, and the pearl oyster is the white butterfly oyster, which is also intact and the age is 1 year.

[0025] (2) Sectioning: tear off the mantle from the pearl oyster, and place it in the mixed solution containing penicillin streptomycin for 60 minutes. The concentration of penicillin in the mixed solution is 1800 U / mL and the concentration of streptomycin is 2000 U / mL. Then rinse with Hanks solution 4 times to remove blood stains, and then cut into 2mm×3mm pieces.

[0026] (3) Cell culture: Add the small pieces obtained in step (2) to the enzyme solution with a concentration of 60 units / mL for enzymatic digestion, the enzyme used is neutral protease (Dispase), incubate at 37°C for 15 hours, and centrifuge the digestion solution at 800 rpm 6 Minutes, discard the supernatant to obtain pearl oyster mantle cells, and then add serum-containin...

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PUM

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Abstract

The invention provides a method of producing pearls on the basis of pearl shell mantel cell culture. The method includes: cutting mantel of a pearl shell into small slices, scattering to obtain single cells by enzymatic depolymerization, performing primary culture prior to subculturing, centrifuging, preparing solution by obtained cells, quantitatively injecting connective tissue in pearl shell mantel with the solution, placing the pearl shells in a culture area, and culturing for one cycle to obtain pearls.

Description

Technical field [0001] The invention belongs to a method for cultivating pearls, especially a method for producing pearls by culturing pearl oyster mantle cells. Background technique [0002] The method of cultivating pearls can generally be carried out by the technique of implanting small pieces of cells. First, select the donor shells to slice the prepared mantle tissue with the tearing method, and then implant the small pieces into the connective tissue of a certain part of the selected oyster shells. The slices are processed and then sent to the breeding area. At present, the use of this method for breeding requires a large amount of pearl oysters to provide small pieces of mantle. At the same time, it is more harmful to the bred oysters, difficult to recover, resulting in higher mortality, low pearl yield, and the size and color of the cultivated pearls are uneven and high-quality Pearl productivity is low. Summary of the invention [0003] The present invention avoids the ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 郭健纪丽丽宋文东高良军韩丝银王雅颖应知伟
Owner BEIHAI XIUPAI JEWELRY
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