Method of producing pearls on the basis of pearl shell mantel cell culture
A mantle cell and pearl oyster technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of difficult recovery, low pearl production rate, high mortality rate, improve survival rate and pearl yield rate, and reduce damage degree, the effect of reducing the number of
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Embodiment 1
[0013] (1) The choice of pearl oyster and yuzhu oyster: choose the pearl oyster as the Hyriopsis mussel, intact and 1 year old, and the yuzhu oyster as the Hyriopsis mussel, also intact and 1 year old.
[0014] (2) Sectioning: tear off the mantle from the pearl oyster and place it in the mixed solution containing penicillin streptomycin for 40 minutes. The concentration of penicillin in the mixed solution is 2000U / mL and the concentration of streptomycin is 2500U / mL. Then rinse with Hanks solution 3 times to remove blood stains, and then cut into 2mm×3mm pieces.
[0015] (3) Cell culture: Add the small pieces obtained in step (2) to the enzyme solution with a concentration of 60 units / mL for enzymatic digestion. The enzyme used is collagenase. Incubate at 37°C for 12 hours, centrifuge the digestion solution at 800 rpm for 6 minutes, and discard The supernatant was used to obtain pearl oyster mantle cells, and then the culture medium containing serum was added to the obtained cells,...
Embodiment 2
[0018] (1) The choice of pearl oyster and Yuzhu shellfish: choose the pearl oyster as the white butterfly shell, which is intact, and the age is 1 year old, and the Yuzhu shell is the white butterfly shell, which is also intact and the age is 2 years old.
[0019] (2) Sectioning: tear off the mantle from the pearl oyster, and place it in the mixed solution containing penicillin streptomycin for 60 minutes. The concentration of penicillin in the mixed solution is 2000 U / mL and the concentration of streptomycin is 2500 U / mL. Then rinse with Hanks solution 4 times to remove blood stains, and then cut into 2mm×3mm pieces.
[0020] (3) Cell culture: Add the small pieces obtained in step (2) to the enzyme solution with a concentration of 80 units / mL for enzymolysis and digestion. The enzyme used is trypsin. Incubate at 37°C for 15 hours, centrifuge the digestion solution at 800 rpm for 6 minutes, and discard The supernatant was used to obtain pearl oyster mantle cells, and then the cultu...
Embodiment 3
[0024] (1) The choice of pearl oyster and pearl oyster: choose the pearl oyster as the penguin oyster, which is intact, and the age is 0.5 years old, and the pearl oyster is the white butterfly oyster, which is also intact and the age is 1 year.
[0025] (2) Sectioning: tear off the mantle from the pearl oyster, and place it in the mixed solution containing penicillin streptomycin for 60 minutes. The concentration of penicillin in the mixed solution is 1800 U / mL and the concentration of streptomycin is 2000 U / mL. Then rinse with Hanks solution 4 times to remove blood stains, and then cut into 2mm×3mm pieces.
[0026] (3) Cell culture: Add the small pieces obtained in step (2) to the enzyme solution with a concentration of 60 units / mL for enzymatic digestion, the enzyme used is neutral protease (Dispase), incubate at 37°C for 15 hours, and centrifuge the digestion solution at 800 rpm 6 Minutes, discard the supernatant to obtain pearl oyster mantle cells, and then add serum-containin...
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