Quasi-automatic in-vitro detection device and method for measuring HCY (homocysteine) by colorimetric method

Abstract

The invention discloses a quasi-automatic in-vitro detection device and method for measuring HCY (homocysteine) by a colorimetric method. The quasi-automatic in-vitro detection device comprises a detection platform, wherein a photoelectric detection module with temperature control and stirring functions, a mechanical arm, a first loading pump, a second loading pump, a first container for containing a first detection reagent and a second container for containing a second detection reagent are arranged on the detection platform; a cuvette preloaded with the first detection reagent is placed on the photoelectric detection module, and a first sample loading needle for loading the first detection reagent and a second sample loading needle for loading the second detection reagent are respectively arranged on the mechanical arm. According to the quasi-automatic in-vitro detection device, automatic loading of the first detection reagent and the second detection reagent is realized, the problem that a detection result is influenced due to reagent content change caused by volatilization of the first detection reagent is avoided, the labor cost of HCY detection is saved, the manual monitoring link is omitted, and the influence of misoperation in the process of manually dropping the detection reagents on the detection result can be prevented as far as possible.

Description

technical field [0001] The invention relates to in vitro diagnostic equipment, in particular to a quasi-automatic in vitro detection equipment and method for measuring HCY using a colorimetric method. Background technique [0002] Homocysteine ​​(HCY) is a sulfur-containing amino acid produced by the metabolism of methionine. 80% of HCY is bound to protein through disulfide bonds in the blood, and only a small part of free homocysteine ​​participates in the circulation. The level of HCY is closely related to cardiovascular diseases and is an important risk factor for the onset of cardiovascular diseases. The increased Hcy in the blood stimulates the blood vessel wall to cause damage to the arterial vessels, leading to inflammation and plaque formation on the vessel wall, and finally causes cardiac blood flow. stymied. Vitamin B nutritional deficiencies will be accompanied by moderate or mild HCY elevation, which will also increase the risk of heart disease. In recent years...

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Problems solved by technology

[0006] 1) The detection reagent R2 needs to be manually added by the detection personnel. Since the cuvette has a tapered opening at the upper end and a vertical narrow-diameter cavity at the lower end, when manually dropping the detection reagent, it is very easy to drop part of the detection reagent due to the operation error of the detection personnel. On the inner wall of the cuvette, the amount of the detection reagent participating in the reaction is insufficient, thereby affecting the detection result;

[0007] 2) The amount of detection reagent R2 added is generally a few microliters, which requires a high level of skill for the operator. A little carelessness will result in insufficient amount of detection reagent added, which is very easy to produce errors, thus affecting the accuracy of the measurement results

[0008] 3) It takes a period of time for the reaction between the detection reagent R1 and the sample to be detected. When the instrument prompts the operator to add the detection reagent R2, because each operator's operating proficiency or the speed of adding the detection reagent will vary, so this will make the The reaction time between the detection reagent and the sample to be tested is different, resulting in differences between the test results; at the same time, this manual addition method increases the workload of the test personnel, and is prone to monitoring errors that affect the test results

[0009] 4) The detection reagent R1 is usually pre-installed in the cuvette. If it is not used for a long time, it will volatilize to a certain extent, which will reduce the active ingredients of the detection reagent R1, so as to affect the final detection result.

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