Experimental device and method of use for the study of seawater shellfish physiology

Abstract

The invention relates to an experimental apparatus for seawater shellfish physiology research and a using method of the experimental apparatus for the seawater shellfish physiology research. The experimental apparatus for the seawater shellfish physiology research comprises an organic glass steel barrel, a sealing cover is arranged at the top of the organic glass steel barrel, and a hopper, a supporting rack and a collecting bottle are arranged inside the organic glass steel barrel. The hopper comprises an upper hopper port and a lower pipe, wherein a supporting net is arranged at the upper hopper port, and the supporting rack is arranged below the hopper and supports the hopper. The collecting bottle is arranged below the hopper, and the lower end of the hopper stretches into the collecting bottle. According to the experimental apparatus for the seawater shellfish physiology research and the using method of the experimental apparatus for the seawater shellfish physiology research, due to the fact that the experiment condition that environmental parameters such as the same natural seawater temperature and the same bait concentration are created, and a large-sized experimental container and a water bathing condition are utilized on the premise that experimental organisms do not have bait and dissolved oxygen threatening, synchronous measuring of seawater shellfish ingestion and metabolic physiology parameters is achieved, experimental links are reduced, the fact that the experimental organisms are in the active state is ensured, and experimental error is reduced.

Description

technical field [0001] The invention relates to an experimental device for studying the physiology of seawater shellfish and a using method thereof. Background technique [0002] The study of shellfish energetics is an important part of the research on the culture capacity of shallow sea culture system. Shellfish feeding, assimilation, respiration, and excretion are important basic parameters in the study of shellfish energetics, marking the energy utilization level of shellfish in food, and have important reference significance for predicting the growth and development of shellfish. Through the research on shellfish feeding, assimilation, respiration, and excretion and the establishment of shellfish energy budget model on this basis, it is possible to judge the suitability of a sea area for shellfish farming, and provide a basis for choosing a suitable farming sea area and farming model. It also provides the necessary theoretical basis for the breeding capacity of the bree...

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Problems solved by technology

At present, the research methods of feeding physiology and metabolic physiology mainly include hydrostatic method and simulated on-site flow tank method. The hydrostatic method mainly uses a container of a certain volume, which is realized by artificially controlling the temperature of the water body and adding bait. The main disadvantage is that the volume of the experimental container is generally small. The bait source of the experimental organisms is difficult to synchronize with the actual bait concentration of the water body in the open sea area, and the accuracy of the experimental results is low; the simulated field flow method is a widely used research method at present. This method designs the secondary water supply system according to local conditions, and places the experimental organisms separately In the water tank, the flow rate of seawater in the water tank is controlled by the shunt tube, and the feeding behavior of the experimental organisms is studied according to the experimental results before and after the experiment. After the feeding experiment is completed, each water tank is closed to conduct metabolic physiological experiments. This method is better. The water temperature and bait concentration in the sea area are synchronized, but the feeding physiology and metabolic physiology experiments need to be completed in two independent steps, the time span is relatively long, the operation is cumbersome, and the vitality of the experimental organisms is difficult to maintain in a good state all the time. The number of experimental organisms in each flow tank is usually small, and the vitality of the experimental organisms will have a great impact on the experimental results, resulting in a greater chance of the experimental results, and some unreasonable experimental results often appear. In addition, this type of experiment Generally, it needs to be carried out in the experimental base near the water source. The source of the experimental seawater is required to be in-situ seawater from the open sea. At present, most of the reservoirs in the experimental bases have been treated with sand filtration when the water enters. very limited

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