34results about How to "Guaranteed fertility" patented technology

The invention relates to chemical male sterilization, pollen-tube pathway and pollen-mediated transformation technologies, and belongs to the technical field of molecular breeding and gene engineering. The method comprises the following steps of: performing chemical male sterilization treatment on a crop T0 of a budding stage; cutting stigmas of buds; mixing fertile pollen by using the pollen-tube pathway technology, namely using target gene-containing agrobacterium-mediated infection solution (added with 5 percent of sucrose and 500muL/L of SilwetL-77) with OD600 of 0.8; and dipping an absorbent cotton ball in the bacterial solution, coating the bacterial solution to the stigma cut ovaries, performing shading treatment for three days to realize normal growth, and finally collecting selfing seeds. Herbicide resistance screening is performed on the generation T1, polymerase chain reaction (PCR) detection is performed on resistant plants, and positive plants are determined. The method has the characteristics of low cost, simplicity in operation, no need of expensive instruments and equipment, high transformation rate (1 to 5 percent) and the like, is suitable for any flowering plant, and can be applied to large-scale crop genetic transformation.

The invention provides an ecological restoration method for a geological collapse area. The ecological restoration method comprises the following steps that gangue is employed as fillers to fill into a collapse area and then the area is flattened by a bulldozer; the gangue layer is solidly pressed by a road roller; a soil coating layer is paved on the gangue layer; the soil coating layer is formed by 30 to 60wt% of sludge, 30 to 50wt% of gangue and 10 to 30wt% of coal ash; surface soil layer with thickness of 0.3 to 0.5cm is paved on the soil coating layer; the surface soil layer is formed by loess, coal ash, sludge, ureaformaldehyde, bacterial manure and polyacrylamide with proper proportions; and at last, Chinese pines, Populus davidiana and leguminosae trees are mixedly planted on the surface soil layer. By the use of the method, productivity of reconstructed soil can be restored and enhanced in short time, soil environment quality can be improved and soil ecological system can be restored; and the method is simple and easy to operate.

The invention discloses a close-to-nature ecological green restoration method for soil slope in South China and belongs to the technical field of soil slope green restoration. According to the 'close-to-nature' theory, through the research on the existing original ecological nature slope in South China, the plant growth and the community structure are analyzed, plant habitat investigation is carried out, indigenous plants like arbor, shrub and herbaceous plants suitable for growth are screen out and matched, and the plant species ratio is determined after further screening by germination tests; with the relatively high competitiveness of the indigenous plants and natural mature soil is used to mix seeds in sowing, slope greening plants can survive and develop more quickly and maintain a long time by ensuring a certain temperature, humidity and fertility to create a better germination environment, later plants do not need human management after seedling formation, and the maintenance cost is low.

The invention relates to a soil remediation agent for heavy metal pollution as well as an application method and application thereof. The remediation agent comprises phosphate and a pH (Potential of Hydrogen) regulator, wherein the phosphate comprises any one or a combination of at least two of phosphate rock powder, calcium magnesium phosphate fertilizer, bone meal, apatite and tailing phosphaterock; and the pH regulator comprises any one or a combination of at least two of oxalic acid, citric acid and ferrous sulfate. The application method of the soil remediation agent comprises the following steps: mixing the soil remediation agent and fertilizer; commonly applying to soil; and ploughing and adjusting the moisture content of the soil. The phosphate and the pH regulator are reasonablymatched, so that the soil remediation agent is green and economical under the condition that the soil fertility is ensured, and has an extremely excellent lasting remediation effect on alkaline or acidic heavy metal polluted soil.

The invention discloses a Myzus periscae overwintering breed conservation breeding method including the following steps of: (1) performing collection, detoxification, purification and renovation on Myzus periscae, and obtaining robust and disease-free Myzus periscae; (2) inoculating the Myzus periscae on fresh radish sprouts or green vegetable sprouts in a glass greenhouse for breeding in November every year; (3) inoculating the Myzus periscae which is on the fresh radish sprouts or green vegetable sprouts onto tobacco flowers in the glass greenhouse in February of the second year, and breeding the Myzus periscae on the tobacco flowers for 10-15 days; and (4) inoculating the Myzus periscae which is on the tobacco flowers onto bottom leaves of tobacco plants in a plastic shed and performing expanding propagation on the Myzus periscae. According to the invention, the tobacco flowers are used as transitional hosts. The tobacco flowers are the parts of the tobacco plants with the lowest nicotine content. The tobacco flowers contain honeydew, and attract the Myzus periscae to eat, and thus the activity and fertility of the Myzus periscae can be ensured. After the method of the invention is used, the survival rate of the Myzus periscae inoculated onto the tobacco leaves is raised to 70% from 10% in the prior art. The egg laying amount, after the Myzus periscae is inoculated with aphidius gifuensis for 7 days, is 60-70 times of the egg laying amount in the prior art.

The invention relates to compound biological bacterial fertilizer suitable for soil improvement of collapsing erosion land. The compound biological bacterial fertilizer is prepared from the followingcomponents in parts by weight: 1 part of a compound fertilizer body with the ratio of N:P2O5:K2O to be 15:15:15, 34 parts of livestock excrements subjected to composting fermentation, 40 parts of charcoal, 4-12 parts of bacillus subtilis inoculant and 4-12 parts of bacillus mucilaginosus inoculant. The compound biological bacterial fertilizer has the advantages that the nutrient elements of soil are increased, and the water retention capacity of the soil is improved.

The invention discloses an ecological restoration method of a geologic subsidence area. The method comprises the following steps: step I: filling the subsidence area with gangue as a backfill material, bulldozing with a bulldozer, and compacting by a road roller to form a gangue layer; step II: laying a 0.5-1 m thick covering soil layer above the gangue layer; step III: laying a 0.3-0.5 m thick surface soil layer on the covering soil layer; and step IV: planting Chinese pines, populus davidiana dode and leguminous trees on the surface soil layer in an inter-row mixing manner at a mixing ratio of (0.5-1.5):(1-3):(2-8), wherein the covering soil layer comprises 30-60wt% of sludge, 30-50wt% of gangue and 10-30wt% of pulverized fuel ash; and the surface soil layer comprises 40-60wt% of loess, 15-30wt% of pulverized fuel ash, 20-30wt% of sludge, 0.3-0.5wt% of ureaformaldehyde, 0.5-0.8wt% of bacterial manure and 0.4-0.6wt% of polyacrylamide.

The invention discloses efficient yield-increasing biological bacterial fertilizer. The formula of the efficient yield-increasing biological bacterial fertilizer consists of the following raw materials in parts by weight: 30 to 40 parts of organic matter, 30 to 40 parts of cultivating soil, 10 to 12 parts of pure water mixture, 4 to 6 parts of actinomycetes, 3 to 5 parts of nitrogen-fixing bacteria, 6 to 8 parts of silicate bacteria, 2 to 3 parts of bacillus subtilis, 3 to 5 parts of paenibacillus polymyxa, 5 to 7 parts of trichoderma viride, 5 to 7 parts of saccharomycetes and 1 to 3 parts of photosynthetic bacteria. Fermentation is conducted on the basis of the traditional organic fertilizer to form the organic matters, neutralized buffering is conducted through high-quality black soil, the organic matters are dispersed into the black soil, chemical fertilizer is added into the pure water mixture, the action effect of the fertilizer is improved by the high-fertility effect of the low-concentration chemical fertilizer, the fertility of the bacterial fertilizer can be guaranteed, the passivated nutritional components in the soil can be released through the decomposition action of the microbial bacteria and then are completely utilized by crops, and the yield increasing effect is achieved; furthermore, nitrogen fixation is conducted by utilizing the absorption and dissimilation effect of the microorganisms well, so the soil fertility is improved and remained.

The invention provides an interplanting method of chilies and tomatoes, and belongs to the technical field of vegetable planting. The interplanting method comprises the steps of firstly planting the tomatoes, wherein the distance of two adjacent lines of tomato pits is 0.5-1 m, inorganic fertilizer and organic fertilizer are applied into each tomato pit, and firstly tomato seedlings are transplanted into the tomato pits and are watered, so that the water content of soil is 70-80 wt%; planting the chilies after a certain period, chili pits are formed among two adjacent lines of tomato pits, theinorganic fertilizer and the organic fertilizer are applied into each chili pit, and chili seedlings are transplanted into the chili pits. By means of the interplanting method, land resources can beeffectively utilized, when the tomatoes bear fruits, the chilies blossoms, pollens of chili flowers can promote the quick maturity of tomato fruits, the growth cycle of the tomatoes is shortened, thetomato fruits are larger, the yield of the tomatoes is increased, the tomatoes are quickly mature under natural conditions, certain chili output is obtained, and the utilization rate of the land resources is increased.

The invention provides a soil water-retaining agent and a preparation method thereof. The soil water-retaining agent comprises an organic water-retaining agent, sodium alginate, clay and turf, and theorganic water-retaining agent comprises polyacrylamide and one or two or more of potassium polyacrylate, sodium polyacrylate, ammonium polyacrylate, a starch-acrylic acid graft copolymer and a cellulose-acrylic acid graft copolymer. The soil water-retaining agent has the advantages of reasonable water absorption and drainage rates, good water retention effect, flexibleness in adjustment of the soil humidity under a wet or drought condition, loosening of soil, preservation of heat of the soil, reduction of mutual inhibition among elements, improvement of the utilization rate of fertilizers andthe content of organic matters in the soil, and improvement of the resistance of the soil to external pressure.

The invention belongs to the technical field of dredged sediment, and particularly relates to a method for producing a greening substrate through rapid composting of dredged sediment, which comprises the following steps: S1, concentrating; S2, acid adding and stirring; S3, preprocessing; S4, adjusting of the carbon nitrogen ratio; and S5, composting. According to the technology and the system, the high-quality wood which is low in energy consumption, short in production period, uniform and consistent in carbonization degree of the interior and the surface of the wood, high in strength, stable in performance, low in production cost and suitable for mass production is provided.

The invention belongs to the technical field of organic fertilizers, and discloses an organic fertilizer for cultivating flowers and plants. The organic fertilizer is used for solving the problem of low conversion efficiency of farmyard manure. The organic fertilizer disclosed by the invention is prepared from the following raw materials in parts by weight: 40-60 parts of straws, 15-30 parts of rabbit manure, 20-40 parts of human manure, 30-40 parts of plant ash, 10-20 parts of rotten sludge, 14-25 parts of radish peel, 25-50 parts of swine excrement, 7-14 parts of tung oil cake, 10-13 parts of Mali peel, 4-5 parts of tea fruit peel, 3-7 parts of tung fruit peel and 5-8 parts of phosphorus powder. According to the organic fertilizer for cultivating the flowers and the plants, disclosed bythe invention, the conversion efficiency of the organic fertilizer can be increased, so that the fertility of soil is improved.

The invention discloses a livestock epidermis deinsectization device for livestock production. The livestock epidermis deinsectization device structurally comprises a massage convex pad brush cylinder, a liquid injection bent pipe, a water pump block, a liquid injection pipe orifice, a hand-lifting cover block and a liquid storage tank seat. By cooperation of the massage convex pad brush cylinderand the liquid injection bent pipe, epidermis nursing and deinsectization are conducted on young livestock after livestock production, manual early-stage prevention operation is conducted, drainage liquid medicine is matched with a convex arc pad pipe and a pulley frame fan plate to achieve a smearing massage effect, early-stage livestock development obtains immunity derivation, and subsequent deinsectization continuous operation is improved; regional breeding of the ecological environment enables subsequent deinsectization efficiency to be improved, it is guaranteed that the flora carrying amount of the ecological environment is reduced, a wing plate body and a convex arc cushion cap entrainment liquid injection through pipe clamping base are matched with membrane surface permeation to achieve a fluidized bed rolling effect, and the fact that capillary massage degree of the livestock epidermis and deinsectization medicine are close to a smearing effect is guaranteed.

The invention discloses a traditional Chinese medicine preparation for treating the pelvic inflammatory disease. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 10 to 20 g of persicaria capitata, 5 to 15 g of mother chrysanthemum, 5 to 15 g of selfheal, 3 to 10 g of vinegar-processed rhizoma corydalis, 3 to 10 g of rubia cordifolia, 5 to 15 g of rhizoma dioscoreae hypoglaucae, 15 to 25 g of emilia sonchifolia, 5 to 15 g of spreading hedyotis herb, 10 to 20 g of urena lobata, 5 to 15 g of whitebackleaf mallotus root, 5 to 15 g of thlaspi arvense, 10 to 20 g of caulis spatholobi, 5 to 15 g of spina gleditsiae, 5 to 15 g of polygonum cuspidatum and 5 to 15 g of dandelion. The selected medicinal materials are excellent in compatibility and take effects of clearing heat and removing toxicity, promoting circulation and removing stasis, eliminating stagnation and removing symptoms and promoting qi circulation and relieving pain together, so that the traditional Chinese medicine preparation has the effects of resisting symptoms and diminishing inflammation. The traditional Chinese medicine preparation for treating the pelvic inflammatory disease is worthy of clinical popularization and application.

The invention relates to the technical field of pineapple crossbreeding, and particularly discloses a precise pineapple flowering phase regulation and control preparation which comprises a flowering phase advanced regulation and control agent which is an aqueous solution. The flowering phase advanced regulating agent is prepared from the following raw materials in parts by weight: 3 to 8 parts of 6-benzylaminopurine, 1 to 4 parts of gibberellin, 3.5 to 8 parts of monopotassium phosphate, 2 to 7 parts of alginate oligosaccharide, 1 to 4 parts of catechin, 0.5 to 3 parts of calcium chloride, 3 to 8 parts of glucose, 30 to 50 parts of ethanol and 100 to 150 parts of water. The precise pineapple flowering phase regulation and control preparation has the effect of bidirectionally regulating and controlling the pineapple flowering phase, and different varieties can be induced to simultaneously bloom by regulating and controlling the component proportion and concentration. The method is also beneficial to formation and development of pollen, can ensure the fertility and pollen quantity of the pollen after the flowering phase is regulated and controlled, and is beneficial to improving the effect of crossbreeding.

The invention discloses a multifunctional self-management support for raspberry planting. The multifunctional self-management support comprises a connecting rod, a plurality of supporting rods, a water supply pipe, heat absorption pipes, a water storage pipe, a hydraulic cylinder, a first soil loosening module and a second soil loosening module. The multifunctional self-management support for raspberry planting is composed of the plurality of supporting rods, the supporting rods are arranged in a rectangle manner, upper connecting pieces are fixedly installed at the tops of the supporting rods, every two adjacent upper connecting pieces are connected through the connecting rod, the heat absorption pipes are installed in the upper connecting pieces, lower connecting pieces are installed in the middles of the supporting rods, and two adjacent lower connecting pieces at the left and right sides are connected through the water supply pipe. The heat absorption pipes are mounted in the support, and absorb sunlight heat, the heat is converted into into power, so that the soil loosening modules can be driven to operate, and energy is saved. When a soil loosening device operates, an inner pipe can be driven to extend out of an outer pipe, a valve element retreats under the action of an elastic piece and is attached to a sealing table, and due to the fact that power of the soil loosening device is provided by sunlight, the effect of regular irrigation can be achieved.