Method for determining powdery mildew resistance of miniature rosa chinensis
A measurement method and technology of powdery mildew, which is applied in the field of plant resistance research, can solve the problem of unstable measurement results, difficulty in simulating the natural process and conditions of pathogens in the method of inoculation and identification of pathogens, and difficulty in accurately reflecting the resistance of modern rose powdery mildew in measurement results, etc. problems, to achieve the effect of meeting the incidence conditions of powdery mildew and having great practical application value
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Embodiment 1
[0017] (1) Preparation of powdery mildew pathogen carrier: select the self-fertile miniature rose variety w14, which is less than 50cm in plant height, suitable for planting in pots with a pot diameter of 10-15cm, and is very susceptible to powdery mildew, as the powdery mildew pathogen carrier for testing, and select healthy branches of w14 Carry out cutting seedlings, and transplant them into pots after 27 days of cuttings. The size of the pots is 12 cm in diameter, with 4 plants per pot, and the substrate is peat. Afterwards, normal fertilizer and water management and prevention and control of diseases and insect pests will be carried out once after 68 days of potting. Use bactericide again, allow powdery mildew to infect and develop, select the potted flower that is seriously infected with powdery mildew as the powdery mildew pathogen carrier, standby;
[0018] (2) Preparation of the test variety group: Select 54 miniature rose varieties with a plant height of less than 50c...
Embodiment 2
[0022] (1) Preparation of powdery mildew pathogen carrier: choose w14, a miniature rose self-fertile variety w14 with a plant height below 50cm, suitable for planting in pots with a pot diameter of 10-15cm, and highly susceptible to powdery mildew, as the powdery mildew pathogen carrier for testing, and select healthy branches of w14 Carry out cutting seedlings, and transplant them into pots after 25 days of cuttings. The size of the pots is 12cm in diameter, with 4 plants per pot, and the substrate is peat. Afterwards, normal fertilizer and water management and prevention and control of diseases and insect pests will be performed once after 62 days of potting. Use fungicide again, allow powdery mildew to infect and develop, select the potted flower that is seriously infected with powdery mildew as the powdery mildew pathogen carrier, standby;
[0023] (2) Preparation of the test variety group: 57 miniature rose varieties with a plant height of less than 50cm, suitable for plan...
Embodiment 3
[0027] (1) Preparation of powdery mildew pathogen carrier: select the self-fertile miniature rose variety w14, which is less than 50cm in plant height, suitable for planting in pots with a pot diameter of 10-15cm, and is very susceptible to powdery mildew, as the powdery mildew pathogen carrier for testing, and select healthy branches of w14 Carry out cutting seedlings, and transplant them into pots after 30 days of cuttings. The size of the pots is 12cm in diameter, 4 plants per pot, and the substrate is peat. Afterwards, normal fertilizer and water management and prevention and control of diseases and insect pests will be performed once after 63 days of potting. Use bactericide again, allow powdery mildew to infect and develop, select the potted flower that is seriously infected with powdery mildew as the powdery mildew pathogen carrier, standby;
[0028] (2) Preparation of the test variety group: Select 55 miniature rose varieties with a plant height of less than 50cm, suita...
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