Recombinant yeast for detecting environmental estrogen and constructing method and use of recombinant yeast

A technology for recombining yeast and detecting the environment, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, and microbial measurement/inspection, etc., to achieve good repeatability, high sensitivity, and improved sensitivity
CN103555601AInactive Publication Date: 2014-02-05SOUTH CHINA NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA NORMAL UNIVERSITY
Publication Date
2014-02-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses recombinant yeast for detecting environmental estrogen and a constructing method and use of the recombinant yeast. The recombinant yeast comprises a yeast expression plasmid and a yeast report plasmid, wherein the yeast expression plasmid comprises a tanichthys albonubes estrogen receptor gene TERalpha represented by the sequence shown as SEQ ID No.1, and a carrier of the yeast report plasmid is pMP206, and is linked with a tanichthys albonubes vitellogenin gene promoter 2 represented by a sequence shown as SEQ ID No.4. The tanichthys albonubes estrogen receptor is used for constructing the expression plasmid, the tanichthys albonubes vitellogenin gene promoter is linked with the report gene to construct the report plasmid. The tanichthys albonubes is sensitive to the change of the living environment, and is a wonderful fish biological marker for monitoring the environmental estrogen pollution by using a biological method, and the vitellogenin is a good biological marker for the environmental internal-secretion interfering-substance. The estrogen receptor and the vitellogenin promoter of the tanichthys albonubes are used for constructing a recombinant yeast evaluation system, so that the sensitivity can be greatly improved.
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Description

technical field

[0001] The invention belongs to the field of biological detection, and in particular relates to a recombinant yeast for detecting environmental estrogen and its construction method and application. Background technique

[0002] Hormones are high-efficiency bioactive substances secreted by biological endocrine cells, which have the characteristics of low concentration and high potency.

[0003] Environmental hormones refer to certain toxic compounds that exist in the environment, can simulate the action of natural hormones, and have a serious impact on the endocrine system of organisms.

[0004] Environmental estrogen is the most important type of environmental hormones, and it is one of the main causes of reproductive disorders, reproductive system deformities, abnormal development, metabolic disorders, and increased incidence of certain malignant tumors. Due to the variety of environmental estrogens, great harm, wide distribution, and complex mechanism of a...

Claims

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