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Method for screening drought tolerant variety by using rice secondary root difference

A technology for secondary roots and rice seeds, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as screening of difficult rice drought-tolerant materials, and achieve the effects of easy screening and differentiation, simple phenotype, and accurate results

Inactive Publication Date: 2015-05-20
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

However, physiological indicators are susceptible to changes in the rice growth environment and there are many uncertainties in the detection methods, so it is difficult to be directly used in the screening of rice drought-tolerant materials
The plant root system is the basis for the survival of the aboveground part of the plant. Therefore, the study of the plant root system is of great significance for improving the drought resistance of plants. However, the identification method of screening drought-tolerant varieties based on the difference of the secondary root system reflecting the drought-tolerant performance of rice has not been reported yet.

Method used

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  • Method for screening drought tolerant variety by using rice secondary root difference
  • Method for screening drought tolerant variety by using rice secondary root difference
  • Method for screening drought tolerant variety by using rice secondary root difference

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Embodiment Construction

[0029] 1.1 Test material

[0030] The present invention is illustrated by taking the transgenic 3 material containing the CBF3 gene that can significantly improve the drought tolerance of rice and its control transgenic 5 NT material (which is the wild type of transgenic 3) without the CBF3 gene as an example. Provided by the Department of Biological Sciences and Bioinformatics.

[0031] 1.2 Test method

[0032] (1) Disinfection treatment of rice seeds

[0033] Take 100 newly harvested, plump, complete and non-insect-eroded rice seeds of the transgenic 3 material containing the CBF3 gene and its control transgenic 5 NT material without the CBF3 gene, respectively, and put them into different mesh bags. 1.0mol / LHNO 3 After soaking for 30min, wash with distilled water for 3 times, and then wash with 10% H 2 o 2 Treat for 15 minutes, wash with distilled water 3 times, and set aside;

[0034] (2) Germination of rice seeds by filter paper method

[0035] Take...

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Abstract

The invention discloses a method for screening a drought tolerant variety by using the rice secondary root difference. The method comprises the following steps: performing pregermination on to-be-screened rice varieties, transplanting the rice varieties, artificially setting a drought environment condition after the rice grows 2-3 months and enters the early heading period, observing the characteristics of the root system of the secondary root to determine the drought tolerance of the rice varieties. Compared with a physiological and biochemical index verification method, the method for verifying the characteristics of the root system of the secondary root has the advantages that the phenotype is simple, the result is accurate, the rice varieties are easy to screen and distinguish, and the like.

Description

technical field [0001] The invention relates to a method for screening drought-tolerant varieties, in particular to a method for screening drought-tolerant varieties by utilizing the difference of rice secondary roots. technical background [0002] my country is a country that is very short of water resources, and drought is one of the main obstacles affecting rice production. Its main performance is that the water deficit in the sensitive period of heading has the greatest impact on yield. Shao Xiwen (2004) showed that after water stress treatment at the heading stage of rice, the physiological indexes such as free proline and chlorophyll content changed most obviously. However, physiological indicators are susceptible to changes in the rice growth environment and there are many uncertainties in the detection methods, so it is difficult to be directly used in the screening of rice drought-tolerant materials. Plant roots are the basis for the survival of abovegroun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00
Inventor 张瑛罗志祥施伏芝阮新民滕斌吴敬德
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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