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Method for screening drought tolerant variety by using rice secondary root difference

A technology for secondary roots and rice seeds, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of difficult rice drought-tolerant material selection, etc., and achieve the effects of easy screening and distinction, simple phenotype, and accurate results

Inactive Publication Date: 2014-02-12
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

However, physiological indicators are susceptible to changes in the rice growth environment and there are many uncertainties in the detection methods, so it is difficult to be directly used in the screening of rice drought-tolerant materials
The plant root system is the basis for the survival of the aboveground part of the plant. Therefore, the study of the plant root system is of great significance for improving the drought resistance of plants. However, the identification method of screening drought-tolerant varieties based on the difference of the secondary root system reflecting the drought-tolerant performance of rice has not been reported yet.

Method used

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  • Method for screening drought tolerant variety by using rice secondary root difference
  • Method for screening drought tolerant variety by using rice secondary root difference
  • Method for screening drought tolerant variety by using rice secondary root difference

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Embodiment Construction

[0029] 1.1 Test materials

[0030] The present invention takes the transgenic 3 material containing the CBF3 gene that can significantly improve the drought tolerance of rice and the control transgenic 5 NT material (the wild type of transgene 3) without the CBF3 gene as an example for illustration. Provided by the Department of Biological Sciences and Bioinformatics.

[0031] 1.2 Test method

[0032] (1) Disinfection treatment of rice seeds

[0033] Take the transgenic 3 material containing the CBF3 gene and the control transgenic 5 NT material that does not contain the CBF3 gene. 100 rice seeds of different materials from different materials that are newly harvested, full, and intact without insect damage, are put into different net bags and reused. 1.0mol / LHNO 3 After soaking for 30 minutes, wash 3 times with distilled water, and then use 10% H 2 O 2 Treat it for 15 minutes, then wash it with distilled water 3 times before use;

[0034] (2) The filter paper method of ri...

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Abstract

The invention discloses a method for screening a drought tolerant variety by using the rice secondary root difference. The method comprises the following steps: performing pregermination on to-be-screened rice varieties, transplanting the rice varieties, artificially setting a drought environment condition after the rice grows 2-3 months and enters the early heading period, observing the characteristics of the root system of the secondary root to determine the drought tolerance of the rice varieties. Compared with a physiological and biochemical index verification method, the method for verifying the characteristics of the root system of the secondary root has the advantages that the phenotype is simple, the result is accurate, the rice varieties are easy to screen and distinguish, and the like.

Description

Technical field [0001] The invention relates to a method for screening drought-tolerant varieties, in particular to a method for screening drought-tolerant varieties by using rice secondary root differences. technical background [0002] my country is a country with very short water resources, and drought is one of the main obstacles affecting rice production. Its main performance is that water deficit has the greatest impact on yield during the sensitive period of heading. Shao Xiwen (2004) showed that the physiological indexes of free proline and chlorophyll content changed most obviously after water stress treatment at the heading stage of rice. However, physiological indicators are easily affected by changes in rice growth environment and their detection methods, and there are many uncertain factors, so it is difficult to directly use in the selection of rice drought-tolerant materials. Plant roots are the basis for the survival of the aboveground parts of plants. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
Inventor 张瑛罗志祥施伏芝阮新民滕斌吴敬德
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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