Method for purifying plasmin in animal medicine and preparing traditional Chinese medicine composition

A technology for animal medicinal materials and compositions, which is applied in the directions of drug combinations, pharmaceutical formulations, medical raw materials derived from arthropods, etc., can solve the problems of large influence and lack of effective components, and achieve the effect of remarkable effect and simple extraction process.

Active Publication Date: 2014-03-26
JIANGSU TIANZHAO PHARMA
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AI Technical Summary

Problems solved by technology

For a long time, the traditional and conventional preparation methods of ground beetle and ant preparation products are decoction or alcohol reflux extraction, concentration, and drying, and there is a lack of reliable and reasonable process evaluation indicators. At the same time, due to factors such as high temperature The active ingredients have a greater impact. We choose the preparation method of homogenate extraction at room temperature and ultrafiltration interception and concentration, which can greatly retain the active ingredients of animal protein in the medicinal materials.

Method used

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  • Method for purifying plasmin in animal medicine and preparing traditional Chinese medicine composition

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Embodiment 1

[0016] Take 100g of ants, crush them into coarse powder, add 6 times the amount of water to soak, stir until homogenized, take the homogenized liquid in a constant temperature water bath (37°C), stir (100 rpm) for 10 hours, centrifuge, discard the precipitate, and take the centrifuge The liquid is first pre-filtered through a 1 μm pore size membrane, the filtrate is sterilized by filtration through a 0.2 μm pore size membrane, and the sterilized filtrate is then passed through an ultrafilter (cut-off molecular segment 3000-20000), and the concentration ratio of the ultrafiltration is about 1:1 (that is, medicinal materials and medicinal materials liquid volume ratio), and the concentrated solution is freeze-dried into powder for future use. The lyophilized powder was prepared into a solution equivalent to 0.1 g of ants per 1 ml, and the urokinase solution was used as the control solution, and the fibrin plate method was used for determination. The results are shown in Table 1. ...

Embodiment 2

[0019] Get soil beetle 100g, operation process is with embodiment 1, and the results are shown in Table 2.

[0020] sample Ⅰ Urokinase Dissolving circle area(mm2 ) 132.25 136.89

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Abstract

The invention discloses a method for separating and purifying the plasmin component in an animal medicine by a membrane separation technology and preparing a traditional Chinese medicine composition by use of the component. The method comprises the following steps: (1) weighing a proper quantity of ants or ground beeltles, and crushing into coarse powder; (2) soaking the coarse powder in water of which the amount is 6 times that of the coarse powder, and stirring to obtain homogenate; and fetching the homogenate and placing in a constant-temperature water bath, and stirring; (3) centrifuging and discarding the precipitate; (4) filtering the centrifugate with a pore membrane; (5) performing ultrafiltration concentration of the filtrate with an ultrafilter (molecular weight cut-off is 3,000-20,000) until the volume ratio of the medicine to the medicine liquid is about 1:1; and freeze-drying the concentrated liquid into powder for later use; and (6) independently using the freeze-dried powder or matching the freeze-dried powder with other components to obtain a composition for use. The invention provides a new method for separating and purifying the plasmin component in the animal medicine; and the extraction process is simple, and the effect is remarkable.

Description

technical field [0001] The invention relates to a method for separating and purifying plasmin components in animal medicinal materials by membrane separation technology, and using the components to prepare traditional Chinese medicine compositions. technical background [0002] The fibrinolytic system refers to the reaction system in which plasminogen is transformed into plasmin by the action of specific activators, leading to the continuous dissolution of fibrin in the body, mainly including plasminogen, plasmin, plasminogen activator and plasminogen. Lysogen activator inhibitors. [0003] Liver fibrosis is a repair response of the liver to chronic damage caused by various factors, characterized by excessive deposition of extracellular matrix (ECM) in the liver, and the degradation of ECM is regulated by various factors. Recent studies have shown that The plasminogen activation system can participate in the degradation process of ECM by regulating the activity of matrix me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K35/64A61K9/19A61P1/16
Inventor 乐智勇夏培元刘任刘丽玲辛小娜张兵兵
Owner JIANGSU TIANZHAO PHARMA
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