Root canal-periapical complex in vitro model
A periapical and complex technology, applied in bioreactor/fermenter combinations, biochemical instruments, biochemical equipment and methods, etc.
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Embodiment 1
[0033] The establishment of the root canal-periapical complex in vitro model of embodiment 1
[0034] (1) Take a single premolar extracted due to orthodontics, use a curette and an ultrasonic scaler to remove the soft tissue and calculus on the root surface, place it in 2.5-3.0mol / L formaldehyde solution, and store it at 20-30°C for later use , to obtain the isolated tooth 1;
[0035] (2) Take a plurality of penicillin bottles, the penicillin bottle is composed of the bottle body 2 and the rubber stopper 3, dig a hole in the center of the rubber stopper 3 according to the diameter of the neck of the isolated tooth, insert the isolated tooth 1 into the rubber stopper 3 through the hole The interface between the isolated tooth 1 and the rubber stopper 3 is sealed with light-cured flowable resin, and the bottle body 2, the rubber stopper 3 with the isolated tooth 1 fixed thereon, are extinguished at a temperature of 110-125°C and a pressure of 10-20 MPa. bacteria treatment;
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Embodiment 2
[0038] Example 2 Establishment of root canal-periapical complex in vitro model
[0039] (1) Take a single premolar extracted due to orthodontics, use a curette and an ultrasonic scaler to remove the soft tissue and calculus on the root surface, place it in 2.5-3.0mol / L formaldehyde solution, and store it at 20-30°C for later use , to obtain the isolated tooth 1;
[0040] (2) Take a plurality of penicillin bottles, the penicillin bottle is composed of the bottle body 2 and the rubber stopper 3, dig a hole in the center of the rubber stopper 3 according to the diameter of the neck of the isolated tooth, insert the isolated tooth 1 into the rubber stopper 3 through the hole The interface between the isolated tooth 1 and the rubber stopper 3 is sealed with light-cured flowable resin, and the bottle body 2 and the rubber stopper 3 with the isolated tooth 1 fixed are sterilized at a temperature of 110-125°C and a pressure of 10-20 MPa. deal with;
[0041]Randomly select two rubber...
Embodiment 3
[0045] Embodiment 3 simulates no source of infection outside the root canal
[0046] Fifteen in vitro models of the complex established in Example 1 or 2 were used, and 5 models were randomly selected on the first day to detect bacteria in the periapical tissue. If bacteria were detected, it indicated that the model preparation was unsuccessful, and the model was remade; if no bacteria were detected, the remaining 10 models were naturally placed in the air without any treatment, and periapical bacteria were detected on the 21st day. At the same time, two positive control groups were set up: the penicillin vial containing the culture medium was directly exposed to the air. Using polymerase chain reaction (PCR, the primers are 16S rDNA universal primers shared by bacteria: upstream: 5'-AGAGTTTGATCCTGGCTCAG-3'; downstream: 5'-GGTTACCTTGTTACGACTT-3', the fragment length is about 1500bp, provided by Shanghai Sangong Bioengineering Company Synthesis.) Detect whether there are bacte...
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