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Live avian encephalomylitis and henpox combined vaccine

A technology for avian encephalomyelitis and dual vaccine, which is applied in the field of poultry vaccine preparation to achieve the effects of genetic stability, good commercial development prospects and effective immune protection

Active Publication Date: 2015-06-10
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many countries in the world have used live vaccines to immunize most chicken flocks. Domestically, inactivated vaccines have been successfully developed, which can be used in areas where chicken production is started or the use of live vaccines is restricted. At present, there are no live vaccine products in China.

Method used

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  • Live avian encephalomylitis and henpox combined vaccine
  • Live avian encephalomylitis and henpox combined vaccine
  • Live avian encephalomylitis and henpox combined vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Breeding of avian encephalomyelitis virus strain

[0017] Take the brain, pancreas and duodenum of chicks with avian encephalomyelitis, grind them under sterile conditions, dilute them with sterilized normal saline at a ratio of 1:5, repeat freezing and thawing three times, centrifuge at 4000rpm for 30 minutes, and take the supernatant After filtering through the liquid filter, 10 1-day-old SPF chicks were inoculated in the brain, 0.1ml per chick. After symptoms such as ataxia and head and neck tremors appeared, blood was collected to kill them. Grind under sterile conditions, dilute 1:5 with sterilized saline, freeze-thaw 3 times, centrifuge at 4000rpm for 30 minutes, take the supernatant as the first-generation isolated virus (F1) and save it for later use. The F1 generation was inoculated into the brain of 1-day-old SPF chicks by the same method as above, and the second generation of isolated virus (F2) was prepared. This has been passed on for 4 gener...

Embodiment 2

[0029] Preparation and Application of Example 2 Avian Encephalomyelitis and Fowlpox Dual Live Vaccine

[0030] 1 material

[0031] 1.1 Virus seeds The quailized attenuated strain of fowlpox virus used in the production of avian encephalomyelitis and chickenpox dual live vaccines is identified, preserved and supplied by the China Veterinary Drug Administration; other attenuated fowlpox virus strains can also be used, but fowl brain The attenuated YBF02 strain isolated with the preservation number of CGMCC No. 8505 was selected as the myelitis virus.

[0032] 1.2 Breeding eggs and test chickens

[0033] 1.2.1 SPF eggs were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.

[0034] 1.2. 27-14 days old SPF chicken SPF breeding eggs were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd., and hatched by themselves after purchase, and reared in a negative pressure isolator after hatching.

[0035] 1.3 Other instruments and re...

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Abstract

The invention provides a live avian encephalomylitis and henpox combined vaccine. The combined vaccine consists of an antigen and a protective agent, wherein the antigen comprises henpox virus and avian encephalomylitis virus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.8505. The combined vaccine prepared by the invention can be used for preventing avian encephalomylitis and henpox at the same time to achieve the aim of preventing two viruses with one injection. Furthermore, The avian encephalomylitis virus screened by the invention is an attenuated virus, performs serial passage in an avian body through horizontal transmitted infection, does not have the phenomenon of virulence return, is stable in heredity, and accords with the standard of no virulence return of an avian encephalomylitis attenuated vaccine strain; the prepared vaccine can provide effective immune protection, and has good commercial development prospect.

Description

technical field [0001] The invention belongs to the technical field of poultry vaccine preparation, and in particular relates to a dual live vaccine of avian encephalomyelitis and fowl pox. Background technique [0002] Avian encephalomyelitis (AE) is a viral infectious disease caused by avian encephalomyelitis virus (AEV), which mainly harms chicks under 4 weeks of age, and infects the central nervous system and causes non-suppurative encephalitis as the main pathological feature. Manifested by neurologic symptoms such as ataxia, head and neck tremors, and numbness of the hind digits, especially tremors in the head and neck. Hence, it used to be called "epidemic tremor". After chickens under 4 weeks of age are infected with AEV, the incidence rate is generally 20% to 60%, and the mortality rate is 25% on average, which can also exceed 50%. Adult chicken infection does not show neurological symptoms, but it can cause a transient decline in egg production. The decline range...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/275A61P31/20A61P31/14A61K39/125
Inventor 王红范根成杜元钊宋新宇胡潇宫晓郭伟伟孙健
Owner YEBIO BIOENG OF QINGDAO
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