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Low virulent strain of pig Japanese encephalitis virus

A technology of porcine Japanese encephalitis and attenuated strains, applied in antiviral agents, virus antigen components, inactivation/attenuation, etc., can solve the problem that vaccines cannot meet the needs of epidemic prevention work and cannot completely protect pigs from the invasion of Japanese encephalitis virus and other issues, to achieve good commercial development prospects, effective immune protection, and genetically stable effects

Active Publication Date: 2014-11-05
YEBIO BIOENG OF QINGDAO
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AI Technical Summary

Problems solved by technology

The Institute of Animal Husbandry and Veterinary Medicine of the Hubei Academy of Agricultural Sciences launched a live attenuated vaccine for pigs in 1999. Although the attenuated vaccine has good protection, it cannot completely protect pigs from the Japanese encephalitis virus.
Existing vaccines are far from meeting the needs of epidemic prevention work

Method used

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  • Low virulent strain of pig Japanese encephalitis virus
  • Low virulent strain of pig Japanese encephalitis virus
  • Low virulent strain of pig Japanese encephalitis virus

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Embodiment Construction

[0009] The present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0010] One, the screening of porcine Japanese encephalitis attenuated YBJE strain of the present invention

[0011] 1. BHK-21 cell passage Select the BHK-21 cells that have grown into a good monolayer, wash with PBS solution, add an appropriate amount of maintenance solution, and inoculate the virus content to 10 5.0 TCID 50 / ml Japanese encephalitis virus JEV-SD0601 strain, set at 35~36℃, 5%CO 2 Under the conditions of static culture, after 72 to 96 hours of culture, the virus fluid was harvested when the cells showed obvious lesions, and continuously p...

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Abstract

The invention aims to provide a low virulent strain of a pig Japanese encephalitis virus. The preservation code of the low virulent strain is CGMCC No. 6753. Continuous passage of the low virulent strain on a BHK-21 cell is realized, and a mouse with an inoculated brain is not caused to be attacked by diseases or die. The low virulent strain is passed to 18 generations through cells and is further passed to 5 generations through anthony pigs, and the mouse is still not caused to be attacked by diseases or die. The anthony pigs which are inoculated with the virus are not attacked by diseases, and viruses are not recycled from blood and brain tissues. First farrowing sows which are pregnant for 30 days are inoculated and killed 21 days later after inoculating, fetuses are all healthy and alive, and results of PCR (polymerase chain reaction) tests of the Japanese encephalitis virus on placentas, amniotic fluid and fetus brain tissues show negative. Screened low virulent vaccine of the Japanese encephalitis virus is stable in heredity and conforms to standards, on no reversion to virulence, of low virulent vaccine strains of the pig Japanese encephalitis virus, and finished vaccine can provide effective immune protection and has good commercialized development prospect.

Description

technical field [0001] The invention belongs to the technical field of screening pathogenic microorganisms in poultry and livestock, and in particular relates to an attenuated strain of porcine Japanese encephalitis virus. Background technique [0002] Porcine Japanese encephalitis is a zoonotic acute infectious disease caused by Japanese Encephalitis Virus (JEV) and transmitted by vector mosquitoes. The disease is very harmful to human beings, with high fatality rate and serious sequelae. And my country is the country with the highest incidence rate of Japanese encephalitis, accounting for 80% of the total number of cases in the world. At the same time, Japanese encephalitis is also one of the major diseases that endanger the pig industry. It can cause clinical symptoms such as abortion and stillbirth in pregnant sows, orchitis in boars and death of piglets, and has caused huge economic losses to the sustainable high-yield development of the pig industry. Pigs are one of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/08A61K39/12A61P31/14C12R1/93
Inventor 邹桂荣管清江孙健邹敏刘新文胡潇韩乃君宋新宇
Owner YEBIO BIOENG OF QINGDAO
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