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Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip

A technology of time-resolved fluorescence and spectinomycin, applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems of low sensitivity, detection influence, large batch difference, etc., and achieve the effect of high sensitivity

Inactive Publication Date: 2014-05-14
JIANGSU WISE SCI & TECH DEV
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Problems solved by technology

The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip

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Embodiment Construction

[0034] 1.1 Preparation of quality control microspheres Preparation of quality control microspheres coated with biotin-labeled γ-globulin (BGG).

[0035] (1) Preparation of biotinylated BGG

[0036] with 0.1 M NaCNBH 3 BGG (purchased from Pel-Freez Biological) was prepared into a 10 mg / mL solution, and Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, Product number: B3295) solution to 16.172 mg / mL, add Biotin-X-X-NHS solution to BGG solution according to the amount of 1 mg BBG protein and 5.4 μL Biotin-X-X-NHS solution, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1 M Tris, 0.3 M NaCl, 0.005 M EDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0037] (2) Fluorescent microspheres coated with aldehyde-modified fluorescent microspheres using the above-mentioned labeled proteins

[0038] Add 6.4 μL ...

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Abstract

The invention relates to a preparation method and an application of a spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip. The test strip consists of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper; quick and fast quantitative detection is performed on the antigen to be detected by taking a time-resolved fluorescent microsphere as an immune marker based on the principle of competition law.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of spectinomycin, a preparation method and application thereof. Background technique [0002] Spectinomycin (English name Spectinomycin) is an aminocyclic alcohol compound produced by Streptomyces [0003] Spectabilis is isolated, mainly exists in the tissue and liver of animal products, can damage the eighth cranial nerve, has nephrotoxicity and neuromuscular blocking effect. [0004] In 2002, the European Union promulgated the maximum residue limit of spectinomycin in tissues (residue markers are all in their original form). The maximum residue limits of spectinomycin in muscle, fat, liver and kidney of all food animals are 300 μg / kg, 500 μg / kg, 1000 μg / kg and 1500 μg / kg respectively. The maximum residue limi...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/558G01N33/582G01N33/585
Inventor 杜道林张勇
Owner JIANGSU WISE SCI & TECH DEV
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