Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for researching influence on angiogenesis in brain of rat suffering from cerebral hemorrhage by high pressure oxygen

A technology of angiogenesis and hyperbaric oxygen, applied in the experimental field, can solve problems such as unclear mechanism

Inactive Publication Date: 2014-05-21
XIANGYA HOSPITAL CENT SOUTH UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few domestic and foreign studies on the mechanism of hyperbaric oxygen on angiogenesis in cerebral hemorrhage, and the mechanism is still unclear.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for researching influence on angiogenesis in brain of rat suffering from cerebral hemorrhage by high pressure oxygen
  • Method for researching influence on angiogenesis in brain of rat suffering from cerebral hemorrhage by high pressure oxygen
  • Method for researching influence on angiogenesis in brain of rat suffering from cerebral hemorrhage by high pressure oxygen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] 120 healthy and clean SD rats were randomly divided into sham operation group (group A), model group (group B) and hyperbaric oxygen group (group C), with 40 rats in each group. 8 rats were randomly selected from each group on 4d, 7d, 14d, 21d, and 28d after operation for various tests, 5 rats in each group were subjected to immunohistochemical experiments; 3 rats in each group were subjected to fluorescent quantitative PCR experiments.

[0011] After intraperitoneal anesthesia with 10% chloral hydrate, the rats were fixed on the rat brain stereotaxic instrument, and the model was made by local disinfection according to the principle of aseptic operation;

[0012] The rats in the hyperbaric oxygen group were placed in the animal cabin 3 hours after the model was made, the cabin door was closed tightly, and the oxygen flow rate was uniformly variable at 5-8 L / min to pressurize for 15 minutes. When the pressure in the cabin reached 0.20 MPa, it started Stabilize the press...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for researching angiogenesis influence in the brains of rats suffering from intracerebral hemorrhage caused by hyperbaric oxygen. SD (Sprague Dawley) rats randomly comprise a sham-operation group (group A), a model group (group B) and a hyperbaric oxygen group (group C). The neovascularization condition of brain tissues around hematoma of rats in each group is observed under an HE dyeing microscope by adopting a tissue slice; the protein expression content of HIF1 (Hypoxia Inducible Factor)-alpha and VEGF (Vascular Endothelial cell Growth Factor) in the brains of the rats in each group are detected by adopting an immunohistochemistry analysis method; the expression level of the HIF1-alpha mRNA and VEGFmRNA of the brain tissues of the brains in each group is determined by adopting a fluorescent quantitation RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method.

Description

technical field [0001] The invention relates to a test method, in particular to a method for studying the effect of hyperbaric oxygen on neovascularization in the brain of rats with cerebral hemorrhage. Background technique [0002] Neovascularization after intracerebral hemorrhage (ICH) increases blood and oxygen supply to damaged brain tissue, which is beneficial to brain tissue repair. Hyperbaric oxygen (hyperbaricoxygen, HBO) can increase the oxygen supply of brain tissue, improve microcirculation, promote the regeneration of blood vessels in damaged tissues, and promote the recovery of nerve function. However, there are few domestic and foreign studies on the mechanism of hyperbaric oxygen on angiogenesis in cerebral hemorrhage, and the mechanism is still unclear. Contents of the invention [0003] After intraperitoneal anesthesia with 10% chloral hydrate, the rats were fixed on the rat brain stereotaxic instrument, and the model was made by local disinfection accord...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00G01N33/53G01N21/78C12Q1/68
Inventor 彭争荣
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com